Isothermal strand displacement nucleic acid amplification

Details Isothermal strand displacement nucleic acid amplification Isothermal strand displacement nucleic acid amplification

1997-11-18

2000-07-11

Zitomer, Stephanie

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 536 2433, C12P 1934, C07H 2104

Patent

active

060871334

ABSTRACT:
Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5' exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.

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