Isothermal amplification of nucleic acids

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C435S091100

Reexamination Certificate

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07824890

ABSTRACT:
A process of amplifying a nucleic acid template dependent on partial destruction of primer molecules which have extended onto the template molecule followed by strand invasion of the partially destroyed primer template by a replacement primer. The destruction of the primer molecule may be performed by either endonuclease or exonuclease digestion. A signal generation from the amplified products may be obtained by the use of adaptors capable of binding probe molecules as well as the amplified product.

REFERENCES:
patent: 5824517 (1998-10-01), Cleuziat et al.
patent: 6251639 (2001-06-01), Kurn
patent: 6399309 (2002-06-01), Price
patent: 2004/0058378 (2004-03-01), Kong et al.
patent: 0497272 (1992-08-01), None
patent: 9949081 (1999-09-01), None
patent: 0120035 (2001-03-01), None
patent: 0236821 (2002-05-01), None

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