Isothermal amplification based assay for the detection and...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C435S810000, C536S022100, C536S024300

Reexamination Certificate

active

07074561

ABSTRACT:
The present invention is directed to isothermal transcription based assays for the detection and quantification of alpha-fetoprotein (AFP) mRNA. The present invention is also directed to oligonucleotides for amplifying AFP mRNA and probes for use in the detection and quantification of the amplification product. The present invention is also directed to detecting hepatocellular carcinoma, metastasis thereof and tumor recurrence by analyzing peripheral blood for the presence of AFP mRNA.

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Wong et al. “Circulating Tumor Cell mRNAs in Peripheral Blood from Hepatocellular Carcinoma Patients Under Radiotherapy, Surgical Resection or Chemotherapy: A Quantitative Evaluation,”Cancer Letters, vol. 167, pp. 183-191 (2001).
Wong et al. “Hematogenous Dissemination of Hepatocytes and Tumor Cells after Surgical Resection of Hepatocellular Carcinoma: A Quantitative Analysis,”Clincal Cancer Research, vol. 5, pp. 4021-4027 (1999).
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Poon et al., “Application of Classification Tree and Neural Network Algorithms to the Identification of Serological Liver Marker Profiles for the Diagnosis of Hepatocellular Carcinoma,”Oncology, vol. 61, pp. 275-283 (2001).

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