Isotachophoretic columns

Details Isotachophoretic columns Isotachophoretic columns

1975-08-21

1976-12-21

Mack, John H.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204180R, 324 71R, G01N 2728, G01N 2700

Patent

active

039987191

ABSTRACT:
An improved isotachophoretic column for separating ions in a test sample is described. The column is formed from a main unitary insulating body, in which appropriate passages are formed to define (a) a pair of chambers for holding leading and lagging electrolytes, respectively, (b) a capillary through which ions of the material to be analyzed are passed in the presence of the electrolytes, (c) channels for interconnecting the electrolyte-holding chambers with opposite ends of the capillary, and (d) a channel for injecting the test sample into one end of the capillary. The capillary has a planar profile, with at least one of its parallel boundary walls defined by one region of the unitary body. The other boundary wall of the capillary is either formed from a second region of the unitary body itself, or by a surface of a separate insulating body that is attached to the main body. The successive ions flowing through the capillary are picked up by a suitable detector extending through the main body and into the capillary at a point intermediate its ends, and the resulting signals from the detector are recorded on an isotachophoregram.

REFERENCES:
patent: 3649499 (1972-03-01), Virtanen et al.
patent: 3705845 (1972-12-01), Everaerts
patent: 3869365 (1975-03-01), Sunden

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