Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2011-03-01
2011-03-01
Raghu, Ganapathirama (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S193000, C435S183000, C435S254200, C435S069100, C435S320100, C435S015000, C536S023200
Reexamination Certificate
active
07897369
ABSTRACT:
The present invention provides isolated polynucleotides and isolated polypeptides. The polypeptides of the present invention have isoprenoid wax ester synthase activity or isoprenoid acyl CoA-synthetase activity. The present invention also includes methods of using the polynucleotides and polypeptides of the present invention. For instance, the methods include producing biodiesel and producing wax esters.
REFERENCES:
patent: 4703004 (1987-10-01), Hopp et al.
patent: 4782137 (1988-11-01), Hopp et al.
patent: 5594115 (1997-01-01), Sharma
patent: 5935824 (1999-08-01), Sgarlato
patent: 6165757 (2000-12-01), Ykema et al.
patent: 7118896 (2006-10-01), Kalscheuer et al.
patent: 2008/0171378 (2008-07-01), Keasling et al.
patent: WO 2007/136762 (2007-11-01), None
Broun et al., Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids. Science, 1998, vol. 282: 1315-1317.
Devos et al., Practical limits of function prediction. Proteins: Structure, Function, and Genetics. 2000, vol. 41: 98-107.
Seffernick et al., Melamine deaminase and Atrazine chlorohydrolase: 98 percent identical but functionally different. J. Bacteriol.,2001, vol. 183 (8): 2405-2410.
Whisstock et al., Prediction of protein function from protein sequence. Q. Rev. Biophysics., 2003, vol. 36 (3): 307-340.
Witkowski et al., Conversion of b-ketoacyl synthase to a Malonyl Decarboxylase by replacement of the active cysteine with glutamine. Biochemistry, 1999, vol. 38: 11643-11650.
Guo et al., Protein tolerance to random amino acid change. PNAS., 2004, vol. 101 (25): 9205-9210.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus AJ245436, Accession No. AJ245436.1, “Pseudomonas putidaOCT plasmid alk genes cluster (alkBFGHJKL, alkN and alkST genes) and flanking DNA, strain TF4-1L (GPo1), formerly annotated asPseudomonas oleovorans,” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/5824135>; first page only.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus EF219372, Accession No. EF219372.1, “Marinobacter hydrocarbonoclasticus strain DSM8798 medium-chain acyl-CoA synthetase (acs1) gene, complete cds,” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/126567221>; 3 pgs.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus EF219373, Accession No. EF219373.1, “Marinobacter hydrocarbonoclasticus strain DSM8798 long-chain acyl-CoA synthetase (acs2) gene, complete cds,” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/126567223>; 3 pgs.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus EF219374, Accession No. EF219374.1, “Marinobacter hydrocarbonoclasticus strain DSM8798 long-chain acyl-CoA synthetase (acs3) gene, complete cds,” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/126567225>; 3 pgs.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus EF219375, Accession No. EF219375.1, “Marinobacter hydrocarbonoclasticus strain DSM8798 long-chain acyl-CoA synthetase (acs4) gene, complete cds,” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/126567227>; 3 pgs.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus EF219376, Accession No. EF219376.1, “Marinobacter hydrocarbonoclasticus strain DSM8798 wax ester synthase (ws1) gene, complete cds,” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/126567229>; 3 pgs.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus EF219377, Accession No. EF219377.1, “Marinobacter hydrocarbonoclasticus strain DSM8798 wax ester synthase (ws2) gene, complete cds,” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/126567231>; 3 pgs.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus EF219378, Accession No. EF219378.1, “Marinobacter hydrocarbonoclasticus strain DSM8798 ws3 gene, complete cds,” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/126567233>; 3 pgs.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus EF219379, Accession No. EF219379.1, “Marinobacter hydrocarbonoclasticus strain DSM8798 ws4 pseudogene, complete sequence,” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/126567235>; 2 pgs.
Genbank Accession Number: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenPept Locus ABJ10798, Accession No. ABJ10798.1, “putative AMP-binding protein [Pseudomonas aeruginosa UCBPP-PA14],” [online]. Bethesda, MD [retrieved on Apr. 3, 2009]. Retrieved from the Internet<URL:http://www.ncbi.nlm.nih.gov
uccore/115584783>; 2 pgs.
Akoh et al., “Enzymatic approach to biodiesel production,” Oct. 31, 2007,J. Agric. Food Chem., 55(22):8995-9005. Available online Sep. 29, 2007.
Alvarez et al., “Lipid storage compounds in marine bacteria,”Appl. Microbiol. Biotechnol., 1997, 47:132-139.
Alvarez et al., “Identification of phenyldecanoic acid as a constituent of triacylglycerols and wax ester produced by Rhodococcus opacus PD630,”Microbiol., May 2002, 148(Pt 5):1407-1412.
Antoni et al., “Biofuels from microbes,”Appl. Microbiol. Biotechnol., Nov. 2007, 77(1):23-35. Available online Sep. 22, 2007.
Arora et al., “Promiscuous fatty acyl CoA ligases produce acyl-CoA and acyl-SNAC precursors for polyketide biosynthesis,”J. Am. Chem. Soc., Jul. 6, 2005, 127(26):9388-9389.
Bowie et al., “Deciphering the message in protein sequences: tolerance to amino acid substitutions,”Science, Mar. 16, 1990, 247(4948):1306-1310.
Canoira et al., “Biodiesel from Jojoba oil-wax: transesterification with methanol and properties as a fuel ,”Biomass and Bioenergy, 2006, 30:76-81. Available online Sep. 19, 2005.
Carvalhal et al., “Ethanol production from lactose and whey byEscherichia coliexpressing genes from Zymomonas mobilis,”Revista De Microbiologia, 1996, 27(4):263-267.
Chemler et al., “Biosynthesis of isoprenoids, polyunsaturated fatty acids and flavonoids in Saccharomyces cerevisiae,”Microb. Cell Fact., May 23, 2006, 5(20):9 pgs.
Chisti, “Biodiesel from microalgae beats bioethanol,”Trends Biotechnol., Mar. 2008, 26(3):126-31. Available online Jan. 24, 2008.
Dahlqvist et al., “Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants,”Proc. Natl. Acad. Sci USA, Jun. 6, 2000, 97(12):6487-6492. Available online May 30, 2000.
Daniel et al., “Induction of a novel class of diacylglycerol acyltransferases
Holtzapple Erik K.
Schmidt-Dannert Claudia
Mueting Raasch & Gebhardt, P.A.
Raghu Ganapathirama
Regents of the University of Minnesota
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