Isomaltose synthase-knockout microorganism belonging eumycota

Details Isomaltose synthase-knockout microorganism belonging eumycota Isomaltose synthase-knockout microorganism belonging eumycota

2003-10-20

2008-03-11

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S254300

Reexamination Certificate

active

07341848

ABSTRACT:
It is intended to provide a host microorganism whereby the expression of a gene encoding a target protein can be effectively elevated in the case of transferring the gene. It is also intended to provide a transformant whereby a target protein can be produced at a high efficiency. It is further intended to provide a production process whereby a target protein can be produced at a high productivity. A microorganism which belongs toEumycotaand lacks the major isomaltose synthase. A gene encoding a target protein is transferred into the microorganism to give a transformant. Then the transformant is cultured under conditions allowing the expression of the transferred gene to thereby produce the protein.

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Tani, S., et al.; “In Vivo and in Vitro Analyses of the AmyR Binding Site of theAspergillus nidulans agdAPromoter; Requirement of the CGG Direct Repeat for Induction and High Affinity Binding of AmyR;”Biosci.Biotechnol. Biochem., vol. 65, No. 7, pp. 1568-1574 (2001).
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S. Tani, et al; “In Vivo and in Vitro Analyses of the AmyR Binding Site of theAspergillus nidulans agdAPromoter; Requirement of the CGG Direct Repeat for Induction and High Affinity Binding of AmyR.;”Biosci.Biotechnol.Biochem.; vol. 65; No. 7; 2001; pp. 1568-1574.

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