Isolation tool for viable c-kit expressing cells

Details Isolation tool for viable c-kit expressing cells Isolation tool for viable c-kit expressing cells

2007-10-23

2007-10-23

Woitach, Joseph (Department: 1636)

Chemistry: molecular biology and microbiology

Vector, per se

C435S354000, C435S361000, C435S365000

Reexamination Certificate

active

10497978

ABSTRACT:
A tool for the isolation of c-kit expressing cells is provided. This tool consists of a c-kit plasmid targeting vector which is capable of integrating into the wild type c-kit allele and encodes a chimeric fluorescent protein comprising a nucleolar localization signal such as, TCOF-12, RLP313, RPS254or Fxr2h5. The construct generates a condensed, bright, fluorescent signal that can be localized in living tissue and after dissociation. The construct allows visualization using confocal microscopy and allows automated cell sorting of the dissociated cells using amongst others flow cytometry.

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