Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1993-02-08
1994-05-17
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435201, 435815, C12N 920, C12N 926
Patent
active
053127471
ABSTRACT:
The instant invention relates to the isolation, purification and utilization of sialidase-L, a novel enzyme capable of producing 2,7-anhydro N-acetylneuraminic acid by the selective cleavage of sialic acid-.alpha.2.fwdarw.3D-galactose glycosidic bonds. The instant invention also relates to a method for the preparation of 2,7-anhydro-N-acetylneuraminic acid using sialidase-L. The instant invention also relates to methods for selectively cleaving sialic acid-.alpha.2.fwdarw.3D-galactose glycosidic linkages and providing a means to selectively destroy the selectin ligands for the treatment of selectin-mediated inflammation. The instant invention also relates to methods for using 2,7-anhydro-N-acetylneuraminic acid and sialidase-L for the selective sialylation of acceptor glycosides to form sialic acid-.alpha.2.fwdarw.3D-galactose glycosidic linkages.
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Li Su-Chen
Li Yu-Teh
Halluin Albert P.
Lilling Herbert J.
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