Isolation, purification and cloning of retinol dehydratase

Details Isolation, purification and cloning of retinol dehydratase Isolation, purification and cloning of retinol dehydratase

1997-05-07

1999-07-27

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435232, 4353201, 435 712, 536 232, C12N 1560, C12N 988, C12N 121, C12N 1563

Patent

active

059289317

ABSTRACT:
The present invention relates to retinol dehydratase, the enzyme which synthesizes the retro-retinoid anhydroretinol in the Spodoptera frugiperda insect cell line Sf-21, as well as the DNA molecule encoding that enzyme. The DNA molecule can be incorporated in a DNA expression system and a host for recombinant production of anhydroretinol dehydratase. The isolated retinol dehydratase protein or polypeptide of the present invention can be combined with a pharmaceutically-acceptable carrier or used alone for administration to mammals, particularly humans, for preventing cell growth and/or uncontrolled cell growth in cells which are growth dependent on retinol and/or 14 hydroxy-4, 14-retro-retinol.

REFERENCES:
Buck et al., "Anhydroretinol: A Naturally Occurring Inhibitor of Lymphocyte Physiology," J. Exp. Med., 178:675-80 (1993).
Bhat et al., "Retinoid Metabolism in Spontaneously Transformed Mouse Fibroblasts (Balb/c 3T12-3 Cells): Enzymatic Conversion of Retinol to Anhydroretinol," Journal of Lipid Research, 20:357-62 (1979).
Buck et al., "Intracellular Signaling by 14-Hydroxy-4,14-Retro-Retinol," Science, 254:1654-56 (1991).
Derguini et al., "Spectroscopic Studies of Anhydroretinol, and Endogenous Mammalian and Insect Retro -Retinoid," Angew. Chem. Int. Ed. Engl., 33(18):1837-39 (1994).
Coughtrie et al., "Sulfation of Endogenous Compounds and Xenobiotics-Interactions and Function in Health and Disease," Chemico-Biological Interactions, 92:247-56 (1994).
Yamazoe et al., "Structural Similarity and Diversity of Sulfotransferases," Chemico-Biological Interactions, 92:107-17 (1994).

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