Isolation of SU1, a starch debranching enzyme, the product of th

Details Isolation of SU1, a starch debranching enzyme, the product of th Isolation of SU1, a starch debranching enzyme, the product of th

1995-03-24

1999-06-15

Robinson, Douglas W.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

435 691, 4351723, 4353201, 435419, 536236, 536 241, C12N 1529, C12N 1582, A01H 400, A01H 500

Patent

active

059124130

ABSTRACT:
SU1, a starch debranching enzyme active in maize endosperm (Zea mays), and the cDNA and gene sequences encoding SU1 are disclosed. The amino acid sequence is significantly similar to that of bacterial isoamylases, enzymes that hydrolyze .alpha.-(1.fwdarw.6) glycosidic bonds. Amino acid sequence similarity establishes SU1 as a member of the .alpha.-amylase superfamily of starch hydrolytic enzymes. Also disclosed are antibodies reactive with the SU1 protein, methods of producing antibodies to the SU1 protein, methods of producing fusion proteins including SU1 and methods of producing transgenic plants with a modified su1 gene. The expressed SU1 protein can serve as a replacement for the bacterial and fungal enzymes currently used in the starch processing industry.

REFERENCES:
Pan, David et al., "A Debranching Enzyme Deficiency in Endosperms of the Sugary-1 Mutants of Maize", Plant Physiol. 74: 324-328 (1984).
Creech, Roy G., "Carbohydrate Synthesis in Maize", Advances in Agronomy, 20: 275-322 (1968).
Preiss, Jack, "Biology and Molecular Biology of Starch Synthesis and its Regulation", Oxford Surveys of Plant Molecular and Cell Biology, 7: 59-114 (1991).
Sumner, James B. et al., "The Water-Soluble Polysaccharides of Sweet Corn", Archives of Biochemistry, 4: 7-9 (1944).

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