Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters carbohydrate production in the plant
Reexamination Certificate
2006-02-07
2006-02-07
Fox, David T. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters carbohydrate production in the plant
C800S278000, C800S286000, C435S320100, C435S419000, C536S023200, C536S023600, C536S024500
Reexamination Certificate
active
06995300
ABSTRACT:
SU1, a starch debranching enzyme active in maize endosperm (Zea mays), and the cDNA and genomic sequences encoding SU1 are disclosed. The amino acid sequence is significantly similar to that of bacterial isoamylases, enzymes that hydrolyze α-(1→6) glycosidic bonds. Amino acid sequence similarity establishes SU1 as a member of the α-amylase superfamily of starch hydrolytic enzymes. Also disclosed are antibodies reactive with the SU1 protein, methods of producing antibodies to the SU1 protein, methods of producing fusion proteins including SU1 as well as recombinant SU1 and methods of producing transgenic plants with a modified Su1 gene. The native or expressed SU1 protein can serve as a replacement for the bacterial and fungal enzymes currently used in the starch processing industry.
REFERENCES:
Pan, David et al., “A Debranching Enzyme Deficiency in Endosperms of the Sugary-1 Mutants of Maize”, Plant Physiol. 74: 324-328 (1984).
Creech, Roy G., “Carbohydrate Synthesis in Maize”, Advances in Agronomy, 20: 275-311 (1968).
Preiss, Jack, “Biology and Molecular Biology of Starch Synthesis and its Regulation”, Oxford Surveys of Plant Molecular and Cell Biology, 7: 59-114 (1991).
Sumner, James B. et al., “The Water-Soluble Polysaccharides of Sweet Corn”, Archives of Biochemistry, 4: 7-9 (1994).
James Martha Graham
Myers Alan M.
Fox David T.
Iowa State University & Research Foundation, Inc.
Weingarten Schurgin, Gagnebin & Lebovici LLP
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