Isolation of spider toxins

Details Isolation of spider toxins Isolation of spider toxins

1990-05-15

1994-01-25

Hill, Jr., Robert J.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

25 or more amino acid residues in defined sequence

530855, C07K 1300

Patent

active

052816931

ABSTRACT:
Methods and compositions for blocking Ca.sup.2+ channels within an organism are provided. For example, a toxin was isolated from the Agelenopsis aperta spider. The toxin comprised a 48 amino acid toxin having a molecular weight of approximately 5,274. This toxin was found to block calcium channels within the central nervous system. The Agelenopsis gene responsible for producing this toxin has been identified and cloned. This gene and/or its derivatives provide a mechanism by which the toxin can be produced using recombinant DNA expression technologies.
The present invention further relates to methods of treating neurological diseases by applying the toxins isolated and identified. The toxin may provide beneficial effects on certain neurological conditions including seizures, ischemic-hypoxic CNS damage, and neurodegenerative disorders. It is also found that the toxins are effective as tags in probing calcium channels.

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