Isolation of pseudomonas salicylate hydroxylase and its use for

Details Isolation of pseudomonas salicylate hydroxylase and its use for Isolation of pseudomonas salicylate hydroxylase and its use for

1994-11-07

1996-12-03

Kight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 25, 435805, 435817, 435874, 435876, 435877, 435970, 435975, 436 63, 436 74, 436815, C12Q 134, C12Q 100, C12N 100

Patent

active

055807461

ABSTRACT:
Salicylate hydroxylase isolated from Pseudomonas bacteria can be used to determine the level of salicylate in a body fluid by reacting a sample of the fluid with the enzyme and monitoring the conversion of salicylate to catechol. A method of purifying the enzyme from crude bacterial extract using a salicylate affinity column is also disclosed.

REFERENCES:
patent: 4303409 (1981-12-01), Ogawa et al.
patent: 4394444 (1983-07-01), Cameron et al.
patent: 4416983 (1983-11-01), Roder et al.
patent: 4777132 (1988-10-01), Green et al.
patent: 5185249 (1993-02-01), Arter et al.
patent: 5320946 (1994-06-01), Daniel et al.
Cameron et al, Clin. Chem., vol. 26, No. 7, p. 1057 (Conference Jul. 20-25, 1980).
Yamamoto, S., Vitamins, vol. 48, No. 9, pp. 419-432, 1974.
White-Stevens et al, J. Biol. Chem, vol. 247, pp. 2358-2370., 1972.
Fleischer et al, Meth. in Enzy., vol. 53, pp. 527-543, 1979.

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