Isolation of pseudomonas salicylate hydroxlase and its use for t

Details Isolation of pseudomonas salicylate hydroxlase and its use for t Isolation of pseudomonas salicylate hydroxlase and its use for t

1992-06-05

1994-11-08

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435189, 435817, 435874, 435877, C12Q 126, C12N 902

Patent

active

053626303

ABSTRACT:
Salicylate hydroxylase isolated from Pseudomonas bacteria can be used to determine the level of salicylate in a body fluid by reacting a sample of the fluid with the enzyme and monitoring the conversion of salicylate to catechol. A method of purifying the enzyme from crude bacterial extract using a salicylate affinity column is also disclosed.

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