Isolation of proteins involved in posttranscriptional gene...

Details Isolation of proteins involved in posttranscriptional gene... Isolation of proteins involved in posttranscriptional gene...

2006-07-24

2009-12-15

Mehta, Ashwin (Department: 1638)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S006120

Reexamination Certificate

active

07632936

ABSTRACT:
The present invention includes a method for detecting and isolating sugarcane proteins that interact with the HC-Pro and P1 proteins of SrMV and other proteins involved in gene silencing, particularly in sugarcane. The method uses a two hybrid assay with an HC-Pro, P1, or other silencing-related protein-containing bait protein and a prey protein containing a polypeptide encoded by a DNA molecule in a cDNA library. The method also includes identification of false positives through reverse two-hybrid assays and using in vitro techniques such as farwestern blots or pull down assays where plant physiological conditions may be replicated. Finally, interactions may be confirmed in planta. Some novel proteins used in and discovered using the these methods are also identified. Methods of using viral and plant proteins to regulate silencing in plants such as sugarcane are also discussed.

REFERENCES:
patent: 5939541 (1999-08-01), Vance et al.
patent: 6040496 (2000-03-01), Law et al.
patent: 7001739 (2006-02-01), Mirkov et al.
patent: 2006/0272047 (2006-11-01), Mirkov et al.
patent: 2006/0272052 (2006-11-01), Mirkov et al.
patent: 2006/0275830 (2006-12-01), Mirkov et al.
patent: 2006/0286607 (2006-12-01), Mirkov et al.
patent: 2007/0067880 (2007-03-01), Mirkov et al.
patent: 03/018809 (2002-08-01), None
Anandalakshmi “A Calmodulin-Related Protein that Suppresses Posttranscriptional Gene Silencing in Plants” Science, American Association for the Advancement of Science, vol. 290, No. 5489 (pp. 142-144), Oct. 6, 2000.
Anandalakshmi et al. “A viral suppressor of gene silencing in plants” Proc. Natl. Acad. Sci. USA vol. 95 (pp. 13079-13084), Oct. 1998.
Atreya et al. “Mutational analysis of the helper component-proteinase gene of potyvirus: Effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility” Proc. Natl. Acad. Sci. USA vol. 90 (pp. 11919-11923), Dec. 1993.
Brigneti et al. “Viral pathogenicity determinants are suppressors of transgene silencing inNicotiana benthamiana” The EMBO Journal, vol. 17, No. 22 (pp. 6739-6746), 1998.
Carrington et al. “Expression of potyviral polyproteins in transgenic plants reveals three proteolytic activities required for complete processing” The EMBO Journal, vol. 9, No. 5 (pp. 1347-1353), 1990.
Chuang et al. “Specific and heritable genetic interference by double-stranded RNA inArabidopsis thaliana” PNAS, vol. 97, No. 9, (pp. 4985-4990), Apr. 25, 2000.
Cogoni et al. “Conservation of transgene-induced post-transcriptional gene silencing in plants and fungi” Trends in Plant Science Reviews, vol. 2, No. 11 (pp. 438-443), Nov. 1997.
Cogoni et al. “Gene silencing inNeurospora crassarequires a protein homologous to RNA-dependent RNA polymerase” Nature, vol. 399 (pp. 166-169), May 13, 1999.
Cogoni et al. “Isolation of quelling-defective (qde) mutants impaired in posttranscriptional transgene-induced gene silencing inNeurospora crassa” Proc. Natl. Acad. Sci. USA vol. 94 (pp. 10233-10238), Sep. 1997.
Cogoni et al. “Posttranscriptional Gene Silencing inNeurosporaby a RecQ DNA Helicase” Science, vol. 286 (pp. 2342-2344), Dec. 17, 1999.
Covey et al. “Plants combat infection by gene silencing” Nature, vol. 385 (pp. 781-782), Feb. 27, 1997.
Cronin et al. “Long-Distance Movement Factor: A Transport Function of the Potyvirus Helper Component Proteinase” The Plant Cell, vol. 7 (pp. 549-559), May 1995.
Dagkessamanskaia et al. FEMS Microbiol. Lett. vol. 200 (pp. 53-58), Jun. 2001.
Dalmay et al. “An RNA-Dependent RNA Polymerase Gene inArabidopsisis Required for Posttranscriptional Gene Silencing Mediated by a Transgene but Not by a Virus” Cell, vol. 101 (pp. 543-553), May 26, 2000.
Dehio et al. “Identification of plant genetic loci involved in a posttranscriptional mechanism for meiotically reversible transgene silencing” Proc. Natl. Acad. Sci. USA, vol. 91 (pp. 5538-5542), Jun. 1994.
Depicker et al. “Post-transcriptional gene silencing in plants” Current Opinion in Cell Biology, vol. 9 (pp. 373-382), 1997.
Elmayan et al. “ArabidopsisMutants Impaired in Cosuppression” The Plant Cell, vol. 10 (pp. 1747-1757), Oct. 1998.
Fields et al. “A novel genetic system to detect protein-protein interactions” Nature, vol. 340 (pp. 245-246), Jul. 20, 1989.
Geering et al. “Genetic Diversity Among Banana Streak Virus Isolates from Australia” Database Embl AF215815 (2 pages), Jul. 18, 2000.
Geering et al. “Genetic Diversity Among Banana Streak Virus Isolates from Australia” XP008017774, Phytopathology, vol. 90, No. 8 (pp. 921-927), Aug. 2000.
Gindullis et al. “MAF1, a Novel Plant Protein Interacting with Matriz Attachment Region Binding Protein MFP1, Is Located at the Nuclear Envelope” The Plant Cell, vol. 11 (pp. 1755-1767), Sep. 1999.
Guo et al. “Self-association and mapping of interaction domains of helper component-proteinase of potato A potyvirus” Journal of General Virology, vol. 80 (pp. 1127-1131), 1999.
Hamilton et al. “A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants” Science, vol. 286 (pp. 950-952), Oct. 29, 1999.
Ingelbrecht et al. “Posttranscriptional Gene Silencing in Transgenic Sugarcane Dissection of Homology-Dependent Virus Resistance in a Monocot That Has a Complex Polyploid Genome” Plant Physiology, vol. 119 (pp. 1187-1197), Apr. 1999.
Ingelbrecht et al. “Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation” Proc. Natl. Acad. Sci. USA, vol. 91 (pp. 10502-10506), Oct. 1994.
Kasschau et al. “A Counterdefensive Strategy of Plant Viruses: Suppression of Posttranscriptional Gene Silencing” Cell, vol. 95 (pp. 461-470), Nov. 13, 1998.
Kasschau et al. “Geneome Amplification and Long-Distance Movement Functions Associated with the Central Domain of Tobacco Etch Potyvirus Helper Component-Proteinase” Virology, vol. 228, Article No. VY968368 (pp. 251-262), 1997.
Kennerdell et al. “Use of dsRNA-Mediated Genetic Interference to Demonstrate thatfrizzledandfrizzled2 Act in the Wingless Pathway” Cell, vol. 95 (pp. 1017-1026), Dec. 23, 1998.
Ketting et al. “mut-7 ofC. elegans, Required for Transposon Silencing and RNA Interference, Is a Homolog of Werner Syndrome Helicase and RNaseD” Cell, vol. 99 (pp. 133-141), Oct. 15, 1999.
Kleiman et al. “Functional Interaction of BRCA1-Associated BARD1 with Polyadenylation Factor CstF-50” Science, vol. 285 (pp. 1576-1579), Sep. 3, 1999.
Kohalmi et al. “Identification and characterization of protein interactions using the yeast 2-hybrid system” Plant Molecular Biology Manuari, vol. M1 (pp. 1-30), 1998.
Kong “Complete Nucleotide Sequence and Analysis of the Putative Polyprotein of Maize Dwarf Mosaic Virus Genomic RNA (Bulgarian Isolate)” Archives of Virology, vol. 143, No. 9 (pp. 1791-1799), 1998.
Kong “Complete Nucleotide Sequence and Analysis of the Putative Polyprotein of Maize Dwarf Mosaic Virus Genomic RNA (Bulgarian Isolate)” Database Embl AJ001691.1 (5 pages), May 6, 1998.
Kumagai et al. “Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA” Proc. Natl. Acad. Sci. USA, vol. 92 (pp. 1679-1683), Feb. 1998.
Kumpatla et al. “Genome intruder scanning and modulation systems and transgene silencing” Trends in Plant Science, vol. 3, No. 3 (pp. 97-104), Mar. 1998.
Lindbo et al. “Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance” The Plant Cell, vol. 5 (pp. 1749-1759), Dec. 1993.
Mallory et al. “Suppression of RNA Silencing in Plants” Phytopathology, vol. 91, No. 6 Supplement (1 page), Jun. 2001.
Matzke et al. “Epigenetic silencing

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