Isolation of principal outer membrane protein and antigen of Chl

Details Isolation of principal outer membrane protein and antigen of Chl Isolation of principal outer membrane protein and antigen of Chl

1981-03-03

1984-01-24

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

436543, 436544, 436545, 436546, 436547, 435 7, 260112R, 260112B, G01N 3356, G01N 3358, G01N 3360, C07G 700

Patent

active

044277822

ABSTRACT:
Procedures are presented for isolating the major outer membrane protein of Chlamydia trachomatis. The isolated protein is a species specific antigen which comprises about 60% of the C. trachomatis cell outer membrane structure. The protein has a molecular weight ranging from about 38,000 to 44,000 daltons, with a mean molecular weight of about 39,500 daltons. The protein antigen is purified from C. trachomatis cells by first extracting the cell contents with a mild anionic detergent, preferably sarcosyl, to leave a residue of intact outer cell membranes. These outer cell membranes are then extracted with a strong anionic detergent, preferably sodium dodecyl sulfate, which solubilizes the 39,500 dalton antigen. The antigen is then purified by hydroxlapatite chromatography. The antigen is species specific for Chlamydia trachomatis and may be utilized in assaying Chlamydial infection in mammals.

REFERENCES:
patent: 4118469 (1978-10-01), Caldwell et al.
Taylor-Robinson et al., Lancet, 2 Jun. 1979, pp. 1162-1163.
Schuller et al., Lancet, 6 Jan. 1979, pp. 19-20.
Banks et al., Infection and Immunity, vol. 20, 1978, pp. 864-866.
Caldwell et al., Infection and Immunity, vol. 31, 1981, pp. 1161-1176.
Caldwell et al., J. Immunol., vol. 115, 1975, pp. 969-975.
Caldwell et al., J. Immunol., vol. 118, 1977, pp. 437-441.
Caldwell et al., J. Immunol., vol. 118, 1977, pp. 442-445.

Affiliated with

Also associated with

Rating

Isolation of principal outer membrane protein and antigen of Chl does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolation of principal outer membrane protein and antigen of Chl, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation of principal outer membrane protein and antigen of Chl will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1287922