Isolation of pancreatin

Details Isolation of pancreatin Isolation of pancreatin

1983-12-23

1986-11-18

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Pancreatin

435226, C12N 994, C12N 964

Patent

active

046236244

ABSTRACT:
Highly active pancreatin is obtained by autolysis of an aqueous isopropanol-containing tissue slurry, preferably buffered with sodium bicarbonate, until a test precipitation with aqueous isopropyl alcohol proves positive, and precipitating the batch with a higher concentration of isopropyl alcohol, resulting in a fiber suspension which can be sieved so that the solution obtained permits direct isolation of pancreatin by further increasing the concentration of isopropyl alcohol. A high bulk density of the finished dry preparation is achieved by stirring the precipitated pancreatin with isopropyl alcohol or acetone so as to bring the pancreatin to a concentration of 70-85% of isopropanol or 80-95% of acetone, isolating the pancreatin by suction filtration or centrifuging and drying it by treatment with dry air or nitrogen.

REFERENCES:
patent: 3223594 (1965-12-01), Hoek et al.
patent: 4088539 (1978-05-01), Muller

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