Isolation of nucleic acid

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C536S025320

Reexamination Certificate

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10820423

ABSTRACT:
Provided is a preparative method for isolating RNA comprising an oligo-or polynucleotide from a sample, which method comprises: (a) treating the sample with a reactant capable of covalently modifying the 2′-OH position of the ribose rings of the RNA under conditions so that a proportion of the 2′-OH positions of the ribose rings bear a substituent; and (b) preparing isolated RNA therefrom by separating material containing the substituent from the sample on the basis of a property of the substituent.

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