Isolation of microbial protein with reduced nucleic acid content

Details Isolation of microbial protein with reduced nucleic acid content Isolation of microbial protein with reduced nucleic acid content

1981-07-09

1982-05-18

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

426656, A23J 118

Patent

active

043304648

ABSTRACT:
The present invention relates to a process for the isolation of protein from microbial cells. The microbial cells are subjected to a treatment to disrupt the cell walls thereby producing a cellular debris fraction and a fraction containing protein and nucleic acid. The two fractions are separated and the fraction containing the protein and nucleic acid is treated with a chelating agent at a pH in the range of 5.5 to 7.0. The resulting solution is passed through an anion exchange column which selectively adsorbs the nucleic acid. The protein is recovered from the resultant eluate.

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