Isolation of lectins

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.

Reexamination Certificate

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C530S370000, C530S501000, C530S333000

Reexamination Certificate

active

07049414

ABSTRACT:
The invention relates to a process for isolating a lectin composition, in particular comprising the mannose-binding lectin (MBL), suitable for using recombinantly produced lectins as starting material, as well as methods for enriching a lectin preparation with respect to high molecular weight lectins. The method implies the use of sugar derivative modified solid supports, wherein the concentration of sugar derivatives on the solid support contributes to the isolation of predetermined oligomers of lectins.

REFERENCES:
patent: WO9106010 (1991-05-01), None
patent: WO0070043 (2000-11-01), None
Haurum, et al., Studies oon the carbohydrate-binding characteristics of human pulmonary surfactant-associated protein A and comparison with two other collections: mannan-binding protein and conglutinin, Biochem. J. vol. 293, pp. 873-878, 1993.
Kenneth et al., Structural Analysis of Monosaccharide Recognition by rat liver mannose-binding protein, J. Biol. Chem., vol. 271, pp 663-674, Jan. 1996.
Blomberg, Lennart Arne, A process for the manufacture of a gel product, EP 0320472 A, Jun. 14, 1989.
Anion exchange resin for sepg. And concentrating boron isotope—contains amino-poly:ol gp., has high sepn. Coefft. And boric acid desorption., Database WPI, Week, Derwent Publications Ltd., London, GB, Class, AN Jun. 7, 1985-174418 & JP 60102947 A, Abstract.
Hydrophilic epoxy resin—by incorporating n-methyl adn/or n-ethyl glucosamine, Database WPO, Week, Derwent Publications ltd., London, GB, Class, AN 1973-21044U & JP 73011239, Jan. 18, 1991, Abstract.
Hartrum, et al., Antibiotic-metal complexes in the detection of gram-negative bacteria and other biological analytes, WO 0127628 A1, Apr. 19, 2001.

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