Isolation of immunoglobulins

Details Isolation of immunoglobulins Isolation of immunoglobulins

2007-10-31

2010-06-29

Russel, Jeffrey E (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

C530S412000, C530S413000, C530S417000

Reexamination Certificate

active

07745582

ABSTRACT:
The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matrices, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.

REFERENCES:
patent: 4043997 (1977-08-01), Schroeder
patent: 4066505 (1978-01-01), Schneider
patent: 4168300 (1979-09-01), Anderssn et al.
patent: 4981961 (1991-01-01), Ngo
patent: 5019269 (1991-05-01), Letourner et al.
patent: 5502022 (1996-03-01), Schwarz et al.
patent: 6498236 (2002-12-01), Lihme et al.
patent: 6919436 (2005-07-01), Lihme et al.
patent: 7442779 (2008-10-01), Lihme et al.
patent: 2005/0053939 (2005-03-01), Chenna et al.
patent: 2007/0274922 (2007-11-01), Wang et al.
patent: 165 912 (1985-12-01), None
patent: 168 363 (1986-01-01), None
patent: 197 521 (1986-10-01), None
patent: 216162 (1987-04-01), None
patent: 242 222 (1987-11-01), None
patent: WO 89/08257 (1989-09-01), None
patent: WO 92/16292 (1992-10-01), None
patent: WO 95/31279 (1995-11-01), None
patent: WO 95/33557 (1995-12-01), None
patent: WO 96/00735 (1996-01-01), None
patent: WO 96/09116 (1996-03-01), None
patent: WO 97/10887 (1997-03-01), None
patent: WO 98/08603 (1998-03-01), None
Beatrix M. Brena et al., “Selective adsorption of immunoglobulins and glucosylated proteins on phenylboronate-agarose”, Journal of Chromatography, 604 (1992) 109-115.
Knudsen et al., “Sulfone-Aromatic Ligands for Thiophilic Adsorption Chromotography: Purification of Human and Mouse Immunoglobulins”, Anal. Biochem, 201, pp. 170-177 (1992).
Porath et al., “Thiophilic Adsorption—A New Method for Protein Fractionation”, FEBS Letters, 185: 306-310 (1985).
Harris et al., “Protein Purification Methods a Practical Approach”, Oxford, IRL Press (1989) pp. 208-211.

Affiliated with

Also associated with

Rating

Isolation of immunoglobulins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolation of immunoglobulins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation of immunoglobulins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4151887