Isolation of human interferon by immunosorbent and high performa

Details Isolation of human interferon by immunosorbent and high performa Isolation of human interferon by immunosorbent and high performa

1982-12-22

1984-11-27

Adee, John

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 2601125R, 435811, B01B 1508

Patent

active

044850174

ABSTRACT:
An improved process for the isolation and purification of HIFNs is disclosed wherein a partially purified preparation of the HIFN is sequentially passed through an antibody affinity column and a reversed-phase high performance liquid chromatographic column. Organic solvents used during the elution are extracted and the protein concentrated for subsequent use.

REFERENCES:
patent: 4125492 (1978-11-01), Cuatrecasas
patent: 4257938 (1981-03-01), Hosoi
patent: 4283199 (1981-08-01), Szabo
patent: 4289189 (1981-09-01), Pestra et al.
patent: 4350760 (1982-09-01), Nicolas et al.
patent: 4359389 (1982-11-01), Heine
Introduction to Modern Liquid Chromatography by Snyder et al., 2nd edition, John Wiley & Sons of N.Y., pp. 285-289, 454 and 455, 1979.

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