Isolation of Hepatitis B surface antigen from transformed yeast

Details Isolation of Hepatitis B surface antigen from transformed yeast Isolation of Hepatitis B surface antigen from transformed yeast

1994-04-28

1995-10-31

Furman, Keith C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

530350, 530395, 530419, 530420, 530421, 530427, 530806, C07K 14085, C07K 130, C07K 136, C12N 1551

Patent

active

054628636

ABSTRACT:
A method for recovering hepatitis B surface antigen protein from transformed yeast cells including the steps of (i) obtaining an aqueous homogenate of the yeast cells; (ii) enriching the hepatitis B surface antigen protein in the homogenate with a protein-aggregating reagent to form a precipitate which contains hepatitis B surface antigen protein; (iii) dissolving the precipitate in a buffer to form a suspension; and (iv) post-homogenizing the suspension to obtain a 10% to 50% increase in yield of the hepatitis B surface antigen protein as calculated based on a yield achieved without performing the post-homogenizing step.

REFERENCES:
patent: 3951937 (1976-04-01), Vnek et al.
patent: 4024243 (1977-05-01), McAleer et al.
patent: 4162192 (1979-07-01), Mizuno et al.
patent: 4335214 (1982-06-01), Adamowicz et al.
patent: 4624918 (1986-11-01), Hershberg
patent: 4707542 (1987-11-01), Friedman et al.
Hitzeman, et al. (1983) "Expression of Hepatitis B Virus Surface Antigen in Yeast", Nucleic Acids Research, 11, 2745-2763.
Kobayashi, et al. (1988) "Recombinant Hepatitis B Virus Surface Antigen Carrying the pre-S2 Region Derived from Yeast", Abstract, DBA Accession No.: 88-10708 from DIALOG file 357, J. Biotechnol., 8, 1-22.
Miyanohara, et al. (1983) "Expression of Hepatitis B Surface Antigen Gene in Yeast", Proc. Natl. Acad. Sci. USA, 80, 1-5.
Valenzuela, et al. (1982) "Synthesis and Assembly of Hepatitis B Virus Surface Antigen Particles in Yeast", Nature, 298, 347-350.
Jendrisak, J. 1987. Jn R. Burgess (ed.), Protein Purification: Micro to Macro, Alan R. Liss, Inc. New York, pp. 75-97.
Bonnerjea et al. 1986. Bio/Technology 4:955,958.

Affiliated with

Also associated with

Rating

Isolation of Hepatitis B surface antigen from transformed yeast does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolation of Hepatitis B surface antigen from transformed yeast , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation of Hepatitis B surface antigen from transformed yeast will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1771757