Isolation of fetal erythrocytes

Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...

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435 725, 435 6, 435962, 436 17, 436175, 436177, 436178, 436518, G01N 33543, G01N 3349, A01N 102

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054570247

ABSTRACT:
A method for isolating nucleated fetal erythrocytes (NFEs) from a maternal blood sample by separating erythrocytes in the maternal blood sample from all other nucleated cells therein using a leukocyte depletion device; and isolating NFEs from nonnucleated maternal erythrocytes. Preferably the maternal blood is peripheral maternal blood. The isolated NFEs can then be analyzed for genetic disorders and the like, such as by in situ hybridization.

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