Isolation of DNA molecules

Details Isolation of DNA molecules Isolation of DNA molecules

2005-07-12

2005-07-12

Leffers, Gerry (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S025410, C536S025600, C536S054000, C528S295000, C528S324000, C528S341000, C522S056000, C210S660000, C210S670000, C210S683000

Reexamination Certificate

active

06916919

ABSTRACT:
The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.

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Lemmens, R., et al. “S-Aryl Ligands for Chromatographic Isolation of Supercoiled Plasmid DNA” 9th Meeting of the European Society of Gene Therapy, Antalya, Turkey Nov. 2-4, 2001, pp. 18-1157-80.

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