Isolation of cells from organ tissue using sonication

Details Isolation of cells from organ tissue using sonication Isolation of cells from organ tissue using sonication

1995-05-17

1999-03-09

Crouch, Deborah

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of detaching cells, digesting tissue or establishing...

435325, 435378, 435380, 435381, C12N 500

Patent

active

058799397

ABSTRACT:
A method for isolating specific viable cell types from surrounding organ tissue is provided. The method entails the use of sonication in conjunction with tissue dissociating agents to free the cells of interest. A specific application of the method is the isolation of the insulin producing tissue of the pancreas, the islets of Langerhans. The method results in a high yield of islets that maintain a high level of viability.

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