Isolation of carboxypeptidase B enzyme

Details Isolation of carboxypeptidase B enzyme Isolation of carboxypeptidase B enzyme

1984-04-02

1987-03-17

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435181, 435226, 435814, 435816, C12N 1108, C12N 1106, C12N 964

Patent

active

046507620

ABSTRACT:
Carboxypeptidase B enzyme is isolated by a process wherein homogenized and autolyzed pancreas is subjected to a thermal treatment, and carboxypeptidase B is separated by fractionation with ammonium sulfate. This process produces carboxypeptidase B having satisfactory specific activity while eliminating the use of pancreas cell fluid or an acetonous powder from pancreas as a starting material. The isolated carboxypeptidase B may be immobilized on a polymer gel containing carboxy groups activated with a carbodiimide.

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Dixon, et al., Enzymes, Academic Press Inc., N.Y., 1964, pp. 27-48.
Marinkovic, et al., Biochem. J., vol. 163, 1977, pp. 253-260.

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