Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1994-04-18
1997-09-09
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435820, 435239, 536 3, 536126, C12N 702, C12N 1106, C08B 3704
Patent
active
056655827
ABSTRACT:
A method for the isolation and sorting of biological materials has been developed. Biological material includes chromosomes, segments of chromosomes, cell organelles, or other minute cellular components. The biological material is separated from the cellular milieu, if necessary, and anchored to a support. Examples of a support are glass coverslips, glass or polymer beads. The anchoring is by means of a reversible polymer and cross-linking system. The supported biological material may then be labelled with compositions capable of binding to said material, and with magnetic particles. Examples of the binding material include nucleic acid probes and antibodies. An example of the antibodies would be those directed to histones. Other labels, for example, fluorescein-biotin-avidin may be used. The material may be released from the support and sorted by a magnetic force. This method is an alternative to flow cytometry and presents numerous advantages in terms of time, resolution, purity, and preservation of the structure of the biological material during isolation and separation.
REFERENCES:
patent: 3970518 (1976-07-01), Glaever
patent: 4454234 (1984-06-01), Czerlinski
patent: 4710472 (1987-12-01), Saur et al.
patent: 4904391 (1990-02-01), Freeman
Cashmore, A., Szabo, L., Timko, M., et al., "Import of Polypeptides into Chloroplasts." Biotechnology, vol. 3 (Sep. 1985), pp. 803-808.
Cooke, H. and Hindley, J., "Cloning of human satellite III DNA; different components are on different chromosomes," Nucleic Acids Research, vol. 6. No. 10 (1979), pp. 3177-3197.
Dahma, M., Giannaris, P., and Zabarylo, S., "An improved procedure for derivatization of controlled-pore glass beads for solid-phase oligonucleotide synthesis," Nucleic Acids Research, vol. 18, No. 13 (1990), pp. 3813-3821.
Dudin, G., Cremer, T., Schardin, M., et al., "A method for nucleic acid hybridization to isolated chromosomes in suspension," Human Genetics, vol. 76 (1987), pp. 290-292.
Dudin, G., Steegmayer, E., Vogt, P., et al., "Sorting of chromosomes by magnetic separation," Human Genetics, vol. 80 (1988), pp. 111-116.
Gengenbach, B., Green, C., and Donovan, C., "Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures," PNAS (USA), vol. 74, No. 11 (Nov. 1977), pp. 5113-5117.
Gruissem, W., Greenberg, B., Zurawski, G., et al., "Biosynthesis of Chloroplast Transfer RNA in a Spinach Chloroplast Transcription System," Cell, vol. 35 (Dec. 1983), pp. 815-828.
Hibberd, K., Walter, T., Green, C., et al., "Selection and Characterization of a Feedback-Insensitive Tissue Culture of Maize," Planta, vol. 148 (1980), pp. 183-187.
Howell, K., Gruenberg, J., Ito, A., et al., "Immuno-Isolation of Subcellular Components," in: Morre, D., et al. (eds). Cell-Free Analysis of Membrane Traffic (New York, Liss. 1988), pp. 77-90.
Hunter, J., Mills, G., and Sturrock, R., "Ferrography--a new method for isolation of particles from biological fluids," Journal of Clinical Pathology, vol. 35 (1982), pp. 689-690.
Kvalheim, G., Fodstad, O., Pihl, A., et al., "Elimination of B-Lymphoma Cells from Human Bone Marrow: Model Experiments Using Monodisperse Magnetic Particles Coated with Primary Monoclonal Antibodies," Cancer Research, vol. 47 (Feb. 1, 1987), pp. 846-851.
Lea, T., Vartdal, F., Davies, C., et al., "Magnetic Monosized Polymer Particles for Fast and Specific Fractionation of Human Mononuclear Cells," Scand. J. Immunol., vol. 22 (1985), pp. 207-216.
Lehman, J., "Brave New Biosensors," Biotechnology, vol. 8 (Aug. 1990), pp. 729-731.
Manning, J., Hershey, N., Broker, T., et al., "A New Method of in situ Hybridization," Chromosoma (Berl.), vol. 55 (1975), pp. 107-117.
Margel, S., Beitler, U., and Ofarim, M., "Polyacrolein Microspheres as a New Tool in Cell Biology," J. Cell Sci., vol. 56 (1982), pp. 157-175.
Margel, S., Zisblatt, S., and Rembaum, A., "Polyglutaraldehyde: A New Reagent for Coupling Proteins to Microspheres and for Labeling Cell-Surface Receptors. II. Simplified Labeling Method by Means of Non-Magnetic and Magnetic Polyglutaraldehyde Microspheres," Journal of Immunological Methods, vol. 28 (1979), pp. 341-353.
Menz, E. Havelick, J., Groman, E., et al., "Magnetic affinity chromatography: An emerging technique," American Biotechnology Laboratory (Sep./Oct. 1986).
Miltenyi, S., Muller, W., Weichel, W., et al., "High Gradient Magnetic Cell Separation with MACS," Cytometry, vol. 11 (1990), pp. 231-238.
Molday, R., Yen, S., and Rembaum, A., "Application of magnetic microspheres in labelling and separation of cells," Nature, vol. 268 (Aug. 4, 1977), pp. 437-438.
Morimoto, Y., Okumura, M., Sugibayashi, K., et al., "Biomedical Applications of Magnetic Fluids. II. Preparation and Magnetic Guidance of Magnetic Albumin Microsphere for Site Specific Drug Delivery In Vivo," J. Pharm. Dyn., vol. 4 (1981), pp. 624-631.
Morimoto, Y., Sugibayashi, K., and Akimoto, M., "Magnetic Guidance of Ferro-colloid-entrapped Emulsion for Site-Specific Drug Delivery," Chem. Pharm. Bull., vol. 31, No. 1 (1983), pp. 279-285.
Mosbach, K., and Anderson,L., "Magnetic ferrofluids for preparation of magnetic polymers and their application in affinity chromatography," Nature, vol. 270 (Nov. 17, 1977), pp. 259-261.
Mosbach, K., and Schroder, U., "Preparation and Application of Magnetic Polymers for Targeting of Drugs," FEBS Letters, vol. 102, No. 1 (Jun. 1979), pp. 112-116.
Narayanswami, S., and Hamkalo, B., "High Resolution Mapping of Xenopus laevis 5S and Ribosomal RNA Genes by EM In Situ Hybridization," Cytometry, vol. 11 (1990), pp. 144-152.
Narayanswami, S., Kausch, A., and Hamkalo, B., "Reversible Immobilization of In-Situ Hybridized Chromosomes. A New Approach to Chromosome Sorting," (1990), 4th Intl. Workshop on Mouse Genome Mapping, Annapolis, MD, Nov. 4-8 (Abstract).
Padmanabhan, R., Corsico, C., Howard, T., et al., "Purification of Transiently Transfected Cells by Magnetic Affinity Cell Sorting," Analytical Biochemistry, vol. 170 (1988), pp. 341-348.
Pain, D., Kanwar, Y., and Blobel, G., "Identification of a receptor for protein import into chloroplasts and its localization to envelopes contact zones," Nature, vol. 331 (Jan. 21, 1988), pp. 232-237.
Rembaum, A. and Dreyer, W., "Immunomicrospheres: Reagents for Cell Labeling and Separation," Science, vol. 208 (Apr. 25, 1980), pp. 364-368.
Richa, J. and Lo, C., "Introduction of Human DNA into Mouse Eggs by Injection of Dissected Chromosome Fragments," Science, vol. 245 (July 14, 1989), pp. 175-177.
Schroder, U., Segren, S., Gemmefors, C., et al., "Magnetic carbohydrate nanoparticles for affinity cell separation," Journal of Immunological Methods, vol. 93 (1986), pp. 45-53.
Senyei, A. and Widder, K., "Drug Targeting: Magnetically Responsive Albumin Microspheres--A Review of the System to Date," Gynecologic Oncology, vol. 12 (1981), pp. 1-13.
Senyei, A., Widder, K., and Czerlinski, G., "Magnetic guidance of drug-carrying microspheres," J. Appl. Phys., vol. 49, No. 6 (Jun. 1978), pp. 3578-3583.
Spangrude, G., Heimfeld, S., and Weissman, I., "Purification and Characterization of Mouse Hematopoietic Stem Cells," Science, vol. 241 (1988), pp. 58-62.
Staros, J., "N-Hydroxysulfosuccinimide Active Esters: Bis(N-hydroxysulfosuccinimide) Esters of Two Dicarboxylic Acids Are Hydrophillic, Membrane-Impermeant, Protein Cross-Linkers," Biochemistry, vol. 21 (1982), pp. 3950-3955.
Uhlen, M., "Magnetic separation of DNA," Nature, vol. 340 (Aug. 31, 1989), pp. 733-734.
Viegas-Pequignot, E., Dutrillaux, B., Magdelenat, H., et al., "Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: Enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy," Proc. Natl. Acad. Sci. USA, vol. 86 (Jan. 1989), pp. 582-586.
Waye, J., Durfy, S., Pinkel, D., et al., "Chromosome-Specific Alpha Satellite DNA from Human Chromosome 1: Hierarchical Structure and Genomic Organization of a Polymorphic Domain Spanning Several Hundred Kilobase Pairs of Centromeric DNA," Genomics, vol. 1 (1987), pp. 43-51.
Widder, K., Senyei, A., Burchette, J.,
Kausch Albert P.
Narayanswami Sandya
Dekalb Genetics Corp.
Fleisher Mindy
Ketter James
LandOfFree
Isolation of biological materials does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Isolation of biological materials, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation of biological materials will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-69083