Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Patent
1986-06-02
1987-11-10
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
530397, 530808, 530825, A61K 3736
Patent
active
047058480
ABSTRACT:
Monomeric, biologically active growth hormone is isolated from microbially-produced insoluble inclusion bodies by solubilizing and denaturing the growth hormone by extraction of the inclusion bodies into a guanidine salt solution such as guanidine hydrochloride and subsequently renaturing at least a portion of the growth hormone in the solution by replacing the guanidine salt solution with a denaturant-free buffer solution and removing precipitated impurities and growth hormone aggregates. The renatured growth hromone is then purified by ion-exchange chromatography.
REFERENCES:
patent: 4511502 (1985-04-01), Builder et al.
patent: 4511503 (1985-04-01), Olson et al.
patent: 4512922 (1985-04-01), Jones et al.
patent: 4518526 (1985-05-01), Olson
Hamilton, Jr. Edwin J.
Taber Larry D.
Yang Ren-Der
Farquer Thomas L.
Guffey Wendell R.
International Minerals & Chemical Corp.
Schain Howard E.
Shearer Peter R.
LandOfFree
Isolation of bioactive, monomeric growth hormone does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Isolation of bioactive, monomeric growth hormone, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation of bioactive, monomeric growth hormone will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1068725