Isolation of a soluble 42 KD pancreatic islet cell autoantigen

Details Isolation of a soluble 42 KD pancreatic islet cell autoantigen Isolation of a soluble 42 KD pancreatic islet cell autoantigen

1993-05-12

1994-05-17

Hill, Jr., Robert J.

Drug, bio-affecting and body treating compositions

Lymphokine

435 721, 530350, 530412, 530416, 530417, 436506, C07K 1506, A61K 3900, G01N 33564

Patent

active

053126289

ABSTRACT:
A method of isolating soluble pancreatic islet antigen employs extraction with a non-ionic detergent and fractionation on a gel filtration column. Fractions are screened for high immunoreactivity with diabetic serum which is positive for ICA immunofluorescence when tested on tissue sections of a pancreas from a human blood group O donor. The isolated soluble antigen is suitable for measuring autoantibodies in both complement fixation assays as well as ELISA-type and immunoblotting formats. Methods of using isolated soluble antigen to test serum for autoantibodies are also taught.

REFERENCES:
patent: 4446240 (1984-05-01), Nerenberg
patent: 4487830 (1984-12-01), Coates et al.
patent: 4855242 (1989-08-01), Soeldner
Baekkeskov, S., et al. (1982) Nature 298: 167-69.
Karounos, D. G. et al. (1988) Diabetes 37, Supp. I: 30A (Abstract #120).
Roep, B. O., et al. (1991) Lancet 337: 1439-41.
McEvoy et al., "A Sensitive Assay to Detect Diabetes-Specific Islet Cell Surface Antibodies (ICSA) Prior to the Onset of Type I Diabetes (IDDM)," Diabetes (1989), vol. 38, suppl. 2, p. 86A, Abst. 343.
Dotta et al., "Immunoreactivity of Islet Tumor Cells: A New Diabetes-Associated Antigen," Clin. Res. (1988), Abstract, vol. 36, p. 480A.
Nayak et al., "Cystoplasmic" Islet Cell Antibodies Evidence that the Target Antigen is a Sialoglycoconjugate Diabetes (1985), 34:617-619.
Baekkeskov et al., "Antibodies to a 64,000 M.sub.r Humman Islet Cell Antigen Precede the Clinical Onset of Insulin-Dependent Diabetes," Clin. Invest. (1987), 79:926-934.
Baekkeskov, et al., Nature, 1990, vol. 347, pp. 151-156.

Affiliated with

Also associated with

Rating

Isolation of a soluble 42 KD pancreatic islet cell autoantigen does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolation of a soluble 42 KD pancreatic islet cell autoantigen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation of a soluble 42 KD pancreatic islet cell autoantigen will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-875329