Isolation of a high molecular weight aortic endothelial cell gro

Details Isolation of a high molecular weight aortic endothelial cell gro Isolation of a high molecular weight aortic endothelial cell gro

1985-07-24

1988-09-13

Rosen, Sam

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

514 2, A61K 3544, A61K 3700

Patent

active

047708778

ABSTRACT:
Liquified vitreous gel, prepared by a non-extractive technique, has been found to contain a cell proliferation inhibitor whose molecular size is greater than 10,000 daltons. Vitreous isolated from both bovine and chick embryo sources has been found to contain such an activity, which inhibits the growth of endothelial cells prepared from calf aorta. Culture medium conditioned by exposure to calf vitreous hyalocytes (cells found on the periphery of the vitreous gel), is also a source of the high molecular weight inhibitor.
The high molecular weight inhibitor is prepared by chromatography of vitreous or hyalocyte-conditioned medium on a column of Bio Gel P-10 or by ultrafiltration. Mateial appearing in the void volume of Bio Gel P-10 (the material whose molecular size is too large to allow it to enter the gel) has a molecular size greater than 13,000 daltons. Ultrafiltration is carried out using a filter whose molecular size cut-off point is 10,000 daltons, therefore material which is retained by the filter has a molecular size of 10,000 daltons and above.

REFERENCES:
patent: Re30239 (1980-02-01), Kuettner et al.
patent: 3864084 (1975-02-01), Folkman
patent: 4042457 (1977-08-01), Kuettner et al.
patent: 4164560 (1979-08-01), Folkman et al.
patent: 4176177 (1979-11-01), Kuettner
patent: 4217412 (1980-08-01), Tolbert et al.
Raymond et al.,-ARVO Abst. (Apr. 1980), Abst 8-10:15, pp. 145-146.
Jacobson et al.-ARVO Abst. (Mar. 1981), Abst. 46, p. 216.
Raymond et al.-Exp. Eye Res., vol. 34.000-00, Feb. 1982.
"Isolation and Identification of Stimulatory and Inhibitory Cell Growth in Bovine Vitreous", by Laurie Raymond et al., Academic Press Inc., 1982, 34. 000-000.
G. A. Lutty et al., J. Cell Sci., 76, 53-65, (Jun. 1985).

Affiliated with

Also associated with

Rating

Isolation of a high molecular weight aortic endothelial cell gro does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolation of a high molecular weight aortic endothelial cell gro, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation of a high molecular weight aortic endothelial cell gro will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-806086