Isolation and uses of caveolae

Details Isolation and uses of caveolae Isolation and uses of caveolae

1996-01-04

1998-07-07

Guzo, David

Chemistry: molecular biology and microbiology

Miscellaneous

521156, 530350, C12N 1102, C08G 1800, C07H 136

Patent

active

057767700

ABSTRACT:
Methods of isolating and purifying caveolae, microdomains of GPI-anchored proteins, and membranes consisting essentially of caveolae associated with microdomains of GPI-anchored proteins from endothelial cell membranes are disclosed. The methods comprise coating a luminal surface of an endothelial cell membrane with an adherent first ionic material by perfusion from a luminal cavity adjacent to the endothelial cell membrane, forming a pellicle by contacting the first ionic material with a second ionic material, and isolating and purifying the pellicle. The pellicle is then processed to isolate the desired cellular component. Caveolae which are substantially free of microdomains of GPI-anchored proteins; microdomains of GPI-anchored proteins which are substantially free of caveolae; and membranes consisting essentially of caveolae, microdomains of GPI-anchored proteins, and caveolae associated with microdomains of GPI-anchored proteins; all of which are substantially free of other cellular elements, are also disclosed.

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