Isolation and use of tissue growth-inducing Frzb protein

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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C536S023500, C435S069100

Reexamination Certificate

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06884871

ABSTRACT:
An isolated cDNA encoding a growth-inducing protein, Frzb, capable of stimulating bone, cartilage, muscle and nerve tissue formation. The CDNA and protein sequences of human and bovine frzb are provided. Production and purification of recombinant Frzb are also described.

REFERENCES:
patent: 5578569 (1996-11-01), Tam
patent: 6133232 (2000-10-01), De Robertis et al.
patent: WO 96 14335 (1996-05-01), None
patent: WO 97 48275 (1997-12-01), None
Adler, P.N., et al. (1990) Molecular Structure of frizzled, a Drosophila Tissue Polarity Gene.Genetics126:401-416.
Aebersold, R. H., et al. (1987) Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.Proc. Natl. Acad. Sci. USA84:6970-6974.
Bhanot, P., et al. (1996) A new member of the frizzled family from Drosophila functions as a Wingless receptor.Nature382:225-230.
Bouwmeester T., et al. (1996) Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer,Nature382:595-601.
Bowie, J. U., et al. (1990) Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions.Science247:1306-1310.
Camac, G., et al. (1996) The homeobox gene Siemois is a target of the Wnt dorsalisation pathway and triggers organiser activity in the absence of mesoderm.Development122:3055-3065.
Chan, S. D. H., et al. (1992) Two Homologs of the Drosophila Polarity Gene frizzled (fz) Are Widely Expressed in Mammalian Tissues.J. Biol Chem.267(35):25202-25207.
Chang, J. T., et al. (1999) Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium .Human Mol. Genetics8(4):575-583.
Chang, S.C., et al. (1994) Cartilage-derived Morphogenetic Proteins.J. Biol. Chem.269(45):28227-28234.
Christian, J. L., et al. (1993) Interactions between Xwnt-8 and Spermann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus.Genes&Development7:13-28.
Cui, Y., et al. (1995) Xwnt-8b: a maternally expressedXenopus Wntgene with a potential role in establishing the dorsoventral axis.Development121:2177-2186.
De Robertis, E. M., et al. (1996) A common plan for dorsoventral patterning in Bilateria.Nature380:37-40.
Epifano, O., et al. (1995) Coordinate expression of the three zona pellucida genes during mouse cogenesis.Development121:1947-1956.
Erlebacher, A., et al. (1995) Toward a Molecular Understanding of Skeletal DevelopmentCell80:371-378.
Finch, P. W., et al. (1997) Purification and moelcular cloning of a secreted. Frizzled-related antagonist of Wnt action.Proc. Natl Acad Sci. USA94:6770-6775.
Harland, R. M. (1991) In Situ Hybridization: An Improved Whole-Mount Method for Xenopus Embryos.Meth. Cell Biol.36:685-695.
Hoang, B., et al. (1996) Primary Structure and Tissue Distribution of FRZB, a Novel Protein Related to Drosophila Frizzled, Suggest a Role in Skeletal Morphogenesis.J. Biol. Chem.271(42):26131-26137.
Hoppler S., et al. (1996) Expression of a dominant-negative Wnt blocks Induction of MyoD in Xenopus embryos.Genes&Development10:2805-2817.
Kao, K. R., et al. (1988) The Entire Mesodermal Mantle Behaves as Spemann's Organizer in Dorsoanterior EnhancedXenopus laevisEmbryos.Develop. Biol.127:64-77.
Kay, B. K. (1991) Injection of Oocytes and Embryos.Methods Cell Biol.36:663-669.
Lemaire, P., et al. (1995) Expression Cloning of Siarnois a Xenopus Homeobox Gene Expressed in Dorsal-Vegetal Cells of Blastulae and Able to Induce a Complete Secondary Axis.Cell81:85-94.
Leyns, L., et al. (1997) Frzb-1 Is a Secreted Antagonist of Wnt Signaling Expressed in the Spemann Organizer.Cell88:747-756.
Luyten, F. P., et al. (1988) Insulin-like Growth Factors Maintain Steady-State Metabolism of Proteoglycans in Bovine Articular Cartilage Explants.Archives of Biochem. and Biophys.267(2):416-425.
Luyten, F. P., et al. (1989) Purification and Partial Amino Acid Sequence of Osteogenin, a Protein Initiating Bone Differentiation.J. Biol. Chem.264(23):13377-13380.
Luyten, F. P., et al. (1994) Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytesin Vitro. Exper. Cell Res.210:224-229.
Marieb, E. N. (1992) In, Human Anatomy and Physiology, The Benjamin/Cummings Publ. Co., 2ndEd., 373-375.
Mayr, T., et al. (1997) Fritz: a secreted frizzled-related protein that inhibits Wnt activity.Mech. Develop.63:09-125.
Melkonyan, H.S., et al. (1997) SARPs: a family of secreted apoptosis-related proteins.Proc. Natl. Acad. Sci. USA94:13636-13641.
Moon, R. T. (1993) In Pursuit of the Functions of the Wnt Family of Developmental Regulators: Insights fromXenopus laevis, BioEssays15(2):91-97.
Moos, M., Jr., et al. (1995) Anti-Dorsalizing Morphogenetic Protein is a novel TGF-β homolog expressed in the Spemann organizer.Development121:4293-4301.
Moos. M., Jr., et al. (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support.J. Biol. Chem.263(13):6005-6008.
Muthukumaran, N., et al. (1985) Comparison of Bone Inductive Proteins of Rat and Porcine Bone Matrix.Biochem. Biophys. Res. Comm.131(1):37-41.
Nardi, J. B., et al. (1976) Polarity and gradients in lepidopteran wing epidermis.J. Embryol. exp. Morph.36(3):489-512.
Nathan, C. and Sporn, M. (1991) Cytokines in Context.J. Cell Biol.113(5):981-986.
Nusse, R. and Varmus, H. E. (1992) Wnt. Genes.Cell69:1073-1087.
Paralkar, V. M., et al. (1989) Affinity of Osteogenin, an Extracellular Bone Matrix Associated Protein Initiating Bone Differentiation, for Concanavalin A.Biochem. Biophys. Res. Comm.160(2):419-424.
Parr, B. A. and McMahon, A. P. (1994) Wnt genes and vertebrate development.Cur. Opin. Genet. Develop.4:523-528.
Pelton, R. W., et al. (1989) Expression of transforming growth factor β2 RNA during murine embryogenesis.Development106:759-767.
Rattner, A., et al. (1997) A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors.Proc. Nat'l. Acad. Sci. USA94:2859-2863.
Richter, K., et al. (1988) Gene expression in the embryonic nervous system ofXenopus laevis. Proc. Natl. Acad. Sci. USA85:8086-8090.
Sambrook, J., et al. (Nov. 1989) Molecular Cloning: A Laboratory Manual Second Edition vols. 1, 2 and 3. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York USA pp. 16.2, 17.2.
Sato, S. M. and Sargent, T. D. (1991) Localized and inducible expression ofXenopus-posterios(Xpo), a novel gene active in early frog embryos, encoding a protein with a ‘CCHC’ finger domain.Development112:747-753.
Scales, J. B., et al. (1990) Two Distinct Xenopus Genes with Homology to MyoD1 Are Expressed before Somite Formation in Early Embryogenesis.Mol. Cell. Biol.10(4):1516-1524.
Shirozu, M., et al. (1996) Characterization of Novel Secreted and Membrane Proteins Isolated by the Signal Sequence Trap Method. Genomics 37:273-280.
Slack, J. M. W. (1994) Inducing factors in Xenopus early embryos.Cur. Biol.4(2):116-126.
Smith, W. C. and Harland, R. M. (1991) Injected Xwnt-8 RNA Acts Early in Xenopus Embryos to Promote Formation of a Vegetal Dorsalizing Center.Cell67:753-765.
Smith, W. C., et al. (1995) A Nodal-Related Gene Defines a Physical and Functional Domain within the Spemann Organizer.Cell82:37-46.
Sokol, S., et al. (1991) Injected Wnt RNA Induces a Complete Body Axis in Xenopus Embryos.Cell67:741-752.
Tempst, P., et al. (1990) Internal sequence analysis of proteins separated on polyacrylamide gels at the submicrogram level: Improved methods, applications and gene cloning strategies.Electrophoresis11:537-553.
Tsukamoto, A. S., et al. (1988) Expression of the int-1 Gene in Transgenic Mice Is Associated with Mammary Gland Hyperplasia and Adenocarcinomas in Male and Female Mice.Cell55:619-625.
van Leeuwen, F. and Nusse, R. (1995) Oncogene activation and oncogene cooperation in MMTV-induced mouse mammary cancer.Cancer Biol.6:127-133.
Vinson, C. R. and Adler, P. N. (1987) Directional non-cell autonomy and the

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