Isolation and use of receptors binding to a peptide column

Details Isolation and use of receptors binding to a peptide column Isolation and use of receptors binding to a peptide column

1990-07-13

1993-04-27

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530395, C07K 318

Patent

active

052063470

ABSTRACT:
A method of isolating cell surface receptors utilizing a short peptide sequence bound to an affinity column. Cell surface receptors which bind selectively to the short peptide and which are specific to various adhesion proteins may be isolated therewith from various cell preparations. These receptors, whose functional integrity has been maintained by the presence of the peptide ligand, are incorporated into liposomes and used to deliver specific compounds inside the liposomes to select tissues containing the specific adhesion proteins.

REFERENCES:
patent: 4988621 (1991-01-01), Ruoslahti et al.
Hansen et al. Biochem J. 201:629-633, 1982.
Cuatrecasas et al. Annu. Rev. Biochem. 40:259-278, 1971.
Alberts et al. Molecular Biology of the Cell, Garland Publishing Inc., New York, N.Y. 1983.
Weiss et al. J. Cell Biol. 88:630-636, 1981.
De Martelaere et al. Cell Biol. Intern. Reports 6(5):489-494, 1982.
Effects of a Serum Spreading Factor on Growth and Morphology of Cells in Serum-Free Medium, David Barnes et al., J. of Supramolecular Structure 14:47-63 (1980).
Cell Attachment on Replicas of SDS Polyacrylamide Gels Reveals Two Adhesive Plasma Proteins, Edward G. Hayman et al., J. of Cell Biology, vol. 95 95 (1982).
Identification and Isolation of a 140 kd Cell Surface Glycoprotein with Properties Expected of a Fibronectin Receptor, R. Pytela et al, Cell, vol. 40 191-198 Jan. 1985.
Dualistic Nature of Adhesive Protein Function; Fibronectin and Its Biologically Active Peptide Fragments Can Autoinhibit Fibronectin Function, K. Yamada et al., J. of Cell Biology vol. 99 Jul. 1984.
Reversible Cross-linking of Cellular Components of Adherent Fibroblasts to Fibronectin and Lectin-Coated Substrata, J. D. Alpin et al. 1981.
Fibronectin and Proteoglycans as Determinants of Cell-Substratum Adhesion, Lloyd A. Culp, et al., J. of Supramolecular Structure 11:401-427 (1979).
Isolation and Purification of Collagen al(1) Receptor from Human Platelet Membrane, Thomas M. Chiang et al., J. of Biological Chemistry vol. 257 (1982).
Monoclonal Antibodies Which Alter the Morphology of Cultured Chick Myogenic Cells, Jeffrey M. Greve et al., J. of Cellular Biochemistry 18:221-229 (1982).
Relationships Between Fibronectin and the Cytoskeleton, Richard O. Hynes (1981) pp. 100-137.
Direction Association of Fibronectin and Actin Molecules in Vitro, Jorma Keski-Oja et al. J. of Cell Biology vol. 85 1980 pp. 527-533.
Isolation of a Collagen-Dependent Cell Attachment Factor, Nature vol. 250 Jul. 1974.
Ganglioside Inhibition of Fibronectin-Mediated Cell Adhesion to Collagen, Hynda Kleinman et al., Proc. Natl. Acad. Set vol. 76, 1976.
Cell Surface Heparan Sulfate Mediates Some Adhesive Responses to Glycosaminoglycan-Binding Matrices, Including Fibronectin, John Laterra et al., J. of Cell Biology vol. 96, 1983.
Thiol-Sensitive Sites in Cell Adhesion, Douglas McAbee et al. Biochem. Journal 1982, 473-478.
A Monoclonal Antibody Detaches Embryonic Skeletal Muscle from Extracellular Matrices, N. T. Neff, et al., J. of Cell Biology vol. 95, Nov. 1982.
Calcium Ions Protect Cell-Substratum Adhesion Receptors Against Proteolysis, N. Oppenheimer-Marks et al., Experimental Cell Research 152 (1984) 467-475.
Plasma Membrane Glycoprotein Which Mediates Adhesion of Fibroblasts to Collagen, Pearlstein, Nature vol. 262, 1976.
Cross-Linking of Fibronectin to Sulfated Proteoglycans at the Cell Surface, Margaret Perkins, et al. Cell, vol. 16, 941-952, 1979.
Interactions Among Heparin, Cold-Insoluble Globulin, and Fibrinogen in Formation of the Heparin-Precipitable Fraction of Plasma, Nicholas E. Statharis, et al., J. of Clinical Investigation vol. 60, 1977.
Cell Surface Molecules and Fibronectin-Mediated Cell Adhesion: Effect of Proteolytic Digestion of Membrane Proteins, Guido Tarone et al., J. of Cell Biology vol. 94, 1982.
Laminin-A Blycoprotein from Basement Membrane, Rupert Timpl et al, J. of Biological Chemistry, vol. 254 (1979).
Isolation of a Cell Surface Receptor Protein for Laminin from Murine Fibrosarcoma Cells, Herbert Malinoff et al., J. of Cell Biology vol. 96, 1983.
Isolation of a Laminin-Binding Protein from Muscle Cell Membranes, Herve Lesot, et al., The EMBO Journal vol. 2, No. 6, 1983.
Isolation and Characterization of a Collagen-Binding Glycoprotein from Chondrocyte Membranes, Jurgen Mollenhauer et al., The EMBO Journal vol. 2, No. 1, 1983.
Role of Collagenous Matrices in the Adhesion and Growth of Cells, Hynda Kleinman et al., J. of Cell Biology vol. 88, 1981.
Synthetic Peptide with Cell Attachment Activity of Fibronectin, Michael Pierschbacher et al., Proc. Natl. Acad. vol. 80, 1983.
Laminin from Rat Yolk Sac Tumor: Isolation, Particle Characterization and Comparison with Mouse Laminin, Eva Engvall et al, Archives of Biochemistry and Biophysics vol. 222, 1983.
Binding of Soluble Form of Fibroblast Surface Protein, Fibronectin, to Collagen, Eva Engvall et al., Int. J. Cancer 20, 1-5, 1977.
Concomitant Loss of Cell Surface Fibronectin and Laminin from Transformed Rat Kidney Cells, Edward Hayman et al, J. of Cell Biology vol. 88, 1981.
Serum Spreading Factor (Vitronectin) is Present at the Cell Surface and in Tissues, Edward Hayman et al., Proc. Natl. Acad. Sci. vol. 80, 1983.
Cell Attachment Activity of Fibronectin Can Be Duplicated by Small Synthetic Fragments of the Molecule, M. D. Pierschbacher et al. Nature, vol. 309, 1984.
Variants of the Cell Recognition Site of Fibronectin that Retain Attachment-Promoting Activity, M. D. Pierschbacher et al. Proc. Natl. Acad. Sci. vol. 81, 1984.
Location of the Cell Attachment Site in Fibronectin with Monoclonal Antibodies and Proteolytic Fragments of the Molecule, M. D. Pierschbacher et al., Cell, vol. 26, 1981.
The Cell Attachment Domain of Fibronectin, M. D. Pierschbacher et al. J. of Biological Chemistry, vol. 257, 1982.
Alignment of Biologically Active Domains in the Fibronectin Molecule, Erkki Ruoslahti et al., J. of Biological Chemistry, vol. 256, 1981.
Interaction of Soluble Fibroblast Surface Antigen with Fibrinogen and Fibrin, Erkki Ruoslahti et al., J. of Experimental Medicine vol. 141, 1975.
Fibronectin: Current Concepts of Its Structure and Functions, Erkki Ruoslahti et al., Coll. Res. vol. 1, 1981.
Localization of the Gangliosides GD2 and GD3 in Adhesion Plaques and on the Surface of Human Melanoma Cells, D. A. Cheresh et al. Proc. Natl. Acad. Sci. vol. 81, 1984.
Inhibition of Human Melanoma Cell Growth in Vitro by Monoclonal Anti-GD3-Ganglioside Antibody, W. G. Dippold et al. Cancer Research 44, Feb. 1984.
Chemical Characterization of a Neural Cell Adhesion Molecule Purified from Embryonic Brain Membranes, S. Hoffman et al., J. of Biological Chemistry, vol. 257, 1982.
Antisera Inhibiting Mammalian Cell Spreading and Possible Cell Surface Antigens Involved, P. Hsieh et al., J. of Cell Biology, 1980.
Membrane Glycoproteins Involved in Cell-Substratum Adhesion, K. A. Knudsen et al., Proc. Natl. Acad. Sci. vol. 78, 1981.
Antibody Affinity May Influence Antigenic Modulation of the Common Acute Lymphoblastic Leukemia Antigen in Vitro, T. W. Lebien et al. J. of Immunology, 1982.
Binding of Plasma Fibronectin to Cell Layers of Human Fibroblasts, P. J. McKeown-Longo et al., J. of Cell Biology, 1983.
New Surface Component of Fibroblast's Focal Contacts Identified by a Monoclonal Antibody, B. Gresch et al., Cell, vol. 31, 1982.
Inhibition of Fibronectin Receptor Function by Antibodies Against Baby Hamster Kidney Cell Wheat Germ Agglutinin Receptors, N. Oppenheimer et al., J. of Cell Biology vol. 95, 1982.
Studies of Cell Adhesion and Recognition I. Extent and Specificity of Cell Adhesion Triggered by Carbohydrate-Reactive Proteins (Clycosidases and Lectins) and by Fibronectin, H. Rauvala, J. of Cell Biology vol. 88, Jan. 1981.
Teratocarcinoma Cell Adhesion: Identification of a Cell-Surface Protein Involved in Calcium-Dependent Cell Aggregation, C. Yoshido et al., Cell, vol. 28, 1982.
Formation of Stress Fibres and Focal Adhesion Sites in Monensin-Exposed Cultured Human Fibroblasts in Response to Exogenously Added Cellular Fibronectin, V. P. Lehto et al., 1985.
Platelet Membrane

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