Isolation and sequencing of the hazel FAD2-Ngene

Details Isolation and sequencing of the hazel FAD2-Ngene Isolation and sequencing of the hazel FAD2-Ngene

1997-03-04

2000-02-15

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 41, 4352523, 4353201, 435325, 435419, 514 44, 536 232, C12N 902, C07H 2104

Patent

active

060251727

ABSTRACT:
The invention relates to the isolation from hazel (Corylus avellana L.) of the FAD2-N gene coding for the .DELTA.12 desaturase enzyme of the microsomal fraction and, in particular, provides the nucleotide sequence and the deduced amino-acid sequence of the gene and provides for the use of the FAD2-N gene as a probe for the isolation of other plant desaturases. The invention also relates to the use of the FAD2-N gene for altering desaturase levels and consequently the fatty-acid composition of the plant.

REFERENCES:
Okuley et al.; The Plant Cell, vol. 6, 147-158, Jan. 1994; "Arabidopsis FAD2 Gene Encodes the Enzyme That Is Essential for Polyunsaturated Lipid Synthesis".
Smith et al.; Nature, vol. 334, 724-726, Aug. 1998; "Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes".
Flavell; Proc. Natl. Acad. Sci. USA, vol. 91, 3490-3496, Apr. 1994; "Inactivation of gene expression in plants as a consequence of specific sequence duplication".
Verwoerd et al.; Nucleic Acids Research, vol. 17, No. 6, 2362, 1989; "A small-scale procedure for the rapid isolation of plant RNAs".
Valenta et al. "Homology of the major birch-pollen allergen, Bet v I, with the major pollen allergens of alder, hazel, and hornbeam at the nucleic acid level as determined by cross-hybridization." Journal of Allergy and Clinical Immunology (Mar. 1991).

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