Isolation and purification pre-S2 containing hepatitis B virus s

Details Isolation and purification pre-S2 containing hepatitis B virus s Isolation and purification pre-S2 containing hepatitis B virus s

1988-09-28

1989-08-08

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 424 89, 530395, 530413, B01D 1508, A61K 3912

Patent

active

048550558

ABSTRACT:
A method for isolating and purifying pre-S2 containing HBsAg from the plasma of a carrier of HBsAg characterized by the use of polymerized human serum albumin (pHSA)-affinity column chromatography.

REFERENCES:
patent: 4639371 (1987-01-01), Prince
patent: 4683136 (1987-07-01), Milich
patent: 4722840 (1988-02-01), Valenzuela
patent: 4742158 (1988-05-01), Lehman
Dean, P. D. G. et al.-Affinity Chromatography, A Practical Approach, pp. 31-32, IRL Press, Oxford, England, 1985.
Neurath, A. R. et al.-Location and Chemical Synthesis of a Pre-S Gene Coded Immunodominant Epitope of Heptatitis B Virus, Science, vol. 224, pp. 392-395, Apr. 27, 1984.
Neurath, A. R. et al.-Hepatitis B. Virus Contains Pre-S Gene-Encoded Domains, Nature, vol. 315, pp. 154-156, May 9, 1985.
Lenkei, R. et al.-Correlations Between Anti-Albumin Antibodies and HB.sub.s AG in Hepatic Patients, Journal of Medical Virology 1, pp. 29-34, 1977.
Machida, A. et al.-A Hepatitis B Surface Antigen Polypeptide (P31) With the Receptor for Polymerized Human as Well as Chimpanzee Albumins, Gastroneterology, vol. 85, No. 2, pp. 268-274, 1983.
Stibbe, W. et al.-Structural Relationships Between Minor and Major Proteins of Hepatitis B Surface Antigen, Journal of Virology, vol. 46, No. 2, pp. 626-628, May 1983.
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Towbin et al.-Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications, Proc. Nat. Acad. Sci., vol. 76, No. 9, pp. 4350-4354, Sep. 1979.
Heermann et al.-Large Surface Proteins of Hepatitis B Virus Containing the Pre-S Sequence, Journal of Virology, vol. 52, pp. 396-402, Nov. 1984.
Michel, M. L. et al.-Expression of Amplified Hepatitis B Virus Surface Antigen Genes in Chinese Hamster Ovary Cells, Biotechnology, vol. 3, pp. 561-567, Jun. 1985.
Yu, M. W. et al.-Interaction Between Various Polymerized Human Albumins and Hepatitis B Surface Antigen, Journal of Virology, vol. 55, pp. 736-743, Sep. 1985.
Laemmli, U. K.-Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature, vol. 227, pp. 680-685, Aug. 15, 1970.
Prince, A. M. et al.-Comparative Evaluations of Hepatitis B Vaccines in Chimpanzees and in Man, pp. 507-523.
Fujisawa, Y. et al.-Expression of Hepatitis B Virus Surface Antigen P31 Gene in Escherichia Coli, Elesvier Science, pp. 23-29, 1985.
Interaction of Hepatitis B Surface Antigen With Polymerized Human Serum Albumin, Journal of Medical Virology, vol. 4, pp. 177-185 (1979).
Hepatitis B Virus Infection, The Western Journal of Medicine, pp. 754-762, May 1984.

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