Isolation and purification of polyribosyl ribitol phosphate from

Details Isolation and purification of polyribosyl ribitol phosphate from Isolation and purification of polyribosyl ribitol phosphate from

1977-12-22

1980-09-02

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435803, 435851, 424 92, 536 1, C12D 1304

Patent

active

042207177

ABSTRACT:
A process for the isolation and purification of immunologically active polyribosyl ribitol phosphate (PRP), the capsular polysaccharide of Haemophilus influenzae type b. The PRP has been purified with ethanol, Cetavlon (hexadecyltrimethyl ammonium bromide) and a phosphate containing adsorbent, hydroxylapatite. The contaminants (nucleic acid, proteins and endotoxins) are removed to the minimum level by a treatment with hydroxylapatite. Also described is a process for the preparation of a combined vaccine containing the PRP (prepared as aforementioned) and B. pertussis antigens. The vaccine elicits anti-PRP antibody and antipertussis antibody (as measured by microagglutination) formations in young animals. This sera with anti-PRP antibody exhibits a strong bactericidal activity.

REFERENCES:
patent: 3978209 (1976-08-01), Limjuco et al.
Rodriques, et al., "Immunity to Haemophilus influenzae type b. I. Isolation, and Some Physiochemical, Serologic and Biologic Properties of the Capsular Polysaccharide of Haemophilus influenzae type b.", Chem. Abst., vol. 75, No. 20, p. 107, Abs. No. 116864n, (1971).
Branefors-Helander, et al., "Structural Studies of the Capsular Antigen from Haemophilus influenza type b.", Chem. Abst., vol. 84, No. 23, p. 309, Abs. No. 162787y, (1976).
Branefors-Helander, et al., "The Structure of the Capsular Antigen from Haemophilus influenza type A.", Chem. Abst., vol. 87, No. 5, p. 345, Abs. No. 37200n, (1977).

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