Isolation and purification of nucleic acids

Details Isolation and purification of nucleic acids Isolation and purification of nucleic acids

2007-09-04

2007-09-04

Strzelecka, Teresa E. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300, C536S025400, C536S025410, C436S177000, C436S178000

Reexamination Certificate

active

10244144

ABSTRACT:
A method for the isolation and purification of nucleic acids such as DNA, RNA, and PNA from various sources using cellulose particles or cellulose paper. Adjusting the concentrations of the salt and polyalkylene glycol to the levels that result in binding of nucleic acids to the cellulose particles or cellulose paper. Separating the nucleic acids bound to the cellulose particles or paper and eluting the nucleic acids from the particles or paper.

REFERENCES:
patent: 4921805 (1990-05-01), Gebeyehu et al.
patent: 5234809 (1993-08-01), Boom et al.
patent: 5437983 (1995-08-01), Watts et al.
patent: 5564104 (1996-10-01), Pourfarzaneh
patent: 5705628 (1998-01-01), Hawkins
patent: 5790964 (1998-08-01), Pourfarzaneh
patent: 5804684 (1998-09-01), Su
patent: 5898071 (1999-04-01), Hawkins
patent: 6027945 (2000-02-01), Smith et al.
patent: 6084091 (2000-07-01), Muller et al.
patent: 6103127 (2000-08-01), Pourfarzaneh
patent: 6416671 (2002-07-01), Pourfarzaneh
patent: 02-289598 (1990-11-01), None
patent: 03-101689 (1991-04-01), None
patent: 07-059572 (1995-03-01), None
patent: WO86/05815 (1986-10-01), None
patent: WO91/12079 (1991-08-01), None
patent: WO96/09379 (1996-03-01), None
patent: WO97/08547 (1997-03-01), None
patent: WO 01/62976 (2001-08-01), None
patent: WO 02/066993 (2002-08-01), None
patent: WO 03/033739 (2003-04-01), None
Ausubel, F. M. et al., “Short Protocols in Molecular Biology”, Third Edition, John Wiley & Sons, Inc., p. 9-52 (1997).
“Extraction and Purification of Plasmid DNA,”Plasmid Vectors, 1.21-1.45.
Weith et al., “Synthesis of Cellulose Derivatives Containing the Dihydroxyboryl Group and a Study of Their Capacity to Form Specific Complexes with Sugars and Nucleic Acid Components,”Biochemistry, 9:4396-4401 (1970).
Shih et al., “Chemical Linkage of Nucleic Acids to Neutral and Phosphorylated Cellulose Powders and Isolation of Specific Sequences by Affinity Chromatography,”Biochemistry, 13:3411-3418 (1974).
Astell et al., “Thermal Elution of Complementary Sequences of Nucleic Acids from Cellulose Columns with Covalently Attached Oligonucleotides of Known Length and Sequence,”J. Biol. Chem., 248:1944-1946 (1971).
Kothari et al., “RNA Fractionation on Modified Celluloses,”J. Chromatogr., 73:449-462 (1972).
Su et al., “Cellulose as a Matrix for Nucleic Acid Purification,”Analytical Biochemistry, 267:415-418 (1999).
“Protocol for Genomic DNA Preparation from Fresh or Frozen Serum for PCR Amplification,”BioTechniques, 29:460-466 (2000).
Ahn et al., “Rapid Mini-Scale Plasmid Isolation for DNA Sequencing and Restriction Mapping,”BioTechniques, 29:466-468 (2000).
Scott et al., “The use of biomagnetic separation to recover DNA suitable for PCR for Claviceps species,”Letters in Applied Microbiology, 31:95-99 (2000).
Taylor, et al., “Application for magnetite and silica-magnetite composites to the isolation of genomic DNA,”Journal of Chromatography A, 890:159-166 (2000).
Levison et al., “Recent developments of magnetic beads for use in nucleic acid purification,”Journal of Chromatography A, 816:107-111 (1998).
Mrázek et al., “Processing of mRNA from Human Leukocytes By Biomagnetical Separation: Comparison with Current Methods of RNA Isolation,”Acta Univ. Palacki. Olomuc., Fac. Med., 142:23-28 (1999).
Davies et al., “Isolation of Plasmid DNA Using Magnetite as a Solid-Phase Adsorbent,”Analytical Biochemistry, 262:92-94 (1998).
Kotsopoulos et al., “Isolation of 3.5-kb Fragments on Magnetic Solid Supports,”BioTechniques, 20:198-200 (1996).
Rudi et al., “Rapid, Universal Method to Isolate PCR-Ready DNA Using Magnetic Beads,”BioTechniques, 22:506-511 (1997).
Harris, A.B., “Solvent pH and salt concentration in rapid resolution of nucleic acid bases on cellulose layers,”Biochem. Biophys. Acta, 145: 520-522 (1967).
Keys et al., “The Use of Cellulose Phosphate in the Extraction of Free Nucleotides from Plant Tissue,”Proceedings of the Biochemical Society, pp. 16p-17p.
Nargessi, R.D., U.S. Appl. No. 60/269,729, filed Feb. 16, 2001, entitled: Magnetic Isolation & Purification of Nucleic Acids.
Nargessi, R.D., U.S. Appl. No. 09/972,752, filed Oct. 5, 2001, entitled: Magnetic Isolation & Purification of Nucleic Acids.
Pourfarzaneh et al., “The Use of Magnetizable Particles in Solid Phase Immunoassay,”Methods of Biochemical Analysis, 1982, vol. 28, pp. 267-295, XP002105006 ISSN: 0076-6941.
Zhang, Yu-ping, et al., “A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay,” XP-002259662,Journal of Virological Methods 71(1998) pp. 45-50.

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