Isolation and purification of lung surfactant protein

Details Isolation and purification of lung surfactant protein Isolation and purification of lung surfactant protein

1990-07-10

1993-11-02

Russel, Jeffrey E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435 691, 435 697, 530359, 530416, 530417, 530424, 530848, C07K 312, C07K 320, C07K 1506, C07K 1516

Patent

active

052584968

ABSTRACT:
Methods are provided for the isolation and purification of recombinant lung surfactant protein. The methods involve a multistep procedure including extraction with a C.sub.1 -C.sub.4 aliphatic alcohol and chromatographic purification steps which employ a C.sub.1 -C.sub.4 aliphatic alcohol as eluant. Purified proteins obtained using the disclosed isolation and purification steps are provided as well.

REFERENCES:
patent: 4882422 (1989-11-01), Taevsch et al.
Scarin et al., J. Lipid Research, vol. 30, No. 4, pp. 607-11, Apr. 1989.
Haagsman et al., Am. J. Physiol., vol. 257 No. 6, Part 1, pp. 6421-6429 (1989).
Phizackerley et al., Biochem J. 183:731-736 (1979).
Tanaka et al., Chem. Pharm. Bull. 31 (11):4091-4099 (1983).
Tanaka et al., Chem. Pharm. Bull. 31(11):4100-4109 (1983).
Tanaka et al., Journal of Lipid Research 27:475-485 (1986).
Whitsett et al., Pediatric Research 20(5):460-466 (1986).
Whitsett et al., Pediatric Research 20(8):744-749 (1986).

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