Isolation and purification of carotenoids from marigold flowers

Organic compounds -- part of the class 532-570 series – Organic compounds – Fatty compounds having an acid moiety which contains the...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C554S008000, C554S012000

Reexamination Certificate

active

07622599

ABSTRACT:
The present invention explains a realistic and effective process for isolating and purifying carotenoids containing higher concentrations of carotenoids such as trans-lutein, trans-zeaxanthin, Cis-lutein, β-carotene and Cryptoxanthin from Marigold flower petals under controlled conditions leaving no traces of any organic hazardous solvents. The process involves ensilaging Marigold flowers, dehydration, solvent extraction, alkali hydrolysis of carotenoid esters with absolute alcohol, crystallization/purification using water, absolute alcohol mixture followed by filteration and drying until the crystals are considerably free from moisture and absolutely free from residual hazardous solvents. These crystals are suitable for nutraceutical and food products as supplements.

REFERENCES:
patent: 5382714 (1995-01-01), Khachik
patent: 5648564 (1997-07-01), Ausich et al.
patent: 6380442 (2002-04-01), Madhavi et al.
patent: 6504067 (2003-01-01), Montoya-Olvera et al.
patent: 6743953 (2004-06-01), Kumar et al.
patent: 7173145 (2007-02-01), Khachik
patent: 1120565 (1996-04-01), None
patent: 11-322708 (1999-11-01), None
patent: 11322708 (1999-11-01), None
patent: 03048284 (2003-06-01), None
Brazana, E. et al., Enzyme-Mediated solvent Extraciton of Carotenoids form Marigold Flower (Tagetes erecta), 2002, Journal of Agric. Food Chem., vo. 50. No. 16, pp. 4491.
Kyowa Hakko Kogyo Co. Ltd. JP 11-322708, 1999, (English translation 8 pages).
Moeller et al., “The Potential Role of Dietary Xanthophyllis in Cataract and Age-Related Macular Degeneration” Journal of the American College of Nutrition, vol. 19, No. 5, 522S-527S (2000).
Hininger et al., “No Significant Effects of Lutein, Lycopene or B-Carotene Supplementation on Biological Markers of Oxidative Stress and LDL Oxidizability in Health Adult Subjects” Journal of the American College of Nutrition, vol. 20, No. 3, 232-238 (2001).
Chopra et al., “Effect of lutein on oxidation of low-density lipoproteins (LDL) in vitro” Proceedings of the Nutrition Society, 53 p 18A.
Britton, “Structure and properties of carotenoids in relation to function” FASEB J 9, 1551-1558 (1995).
Pfander, “Carotenoids: An Overview” Methods In Enzymology, vol. 213 (1992).
Howard et al., “Do Hydroxy-Carotenoids Prevent Coronary Heart Disease? A Comparison Between Belfast and Toulouse” Internat. J. Vit. Nutr. Res. 66, 113-118 (1996).
Chew et al., “Effects of Lutein from Marigold Extract on Immunity and Growth of mammary Tumors in Mice” Anti Cancer Research 16, 3689-3694 (1996).
Ong et al., “Natural Sources of Carotenoids from Plants and Oils” Methods In Enzymology, vol. 213 (1992).
Mercandate, “New carotenoids: Recent progress” Pure Appl. Chem., vol. 71, No. 12, pp. 2263-2272 (1999).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Isolation and purification of carotenoids from marigold flowers does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolation and purification of carotenoids from marigold flowers, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation and purification of carotenoids from marigold flowers will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4144057

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.