Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Reexamination Certificate
2006-05-23
2006-05-23
Romeo, David (Department: 1647)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
C530S350000
Reexamination Certificate
active
07049291
ABSTRACT:
An isolated cDNA encoding a growth-inducing protein, Frzb, capable of stimulating bone, cartilage, muscle and nerve tissue formation. Frzb binds to and modulates the activity of Wnt growth factors which play a role in various developmental and neoplastic processes. The cDNA and protein sequences of human, bovine andXenopusFrzb are provided. Production and purification of recombinant Frzb are also described.
REFERENCES:
patent: 5578569 (1996-11-01), Tam
patent: 6133232 (2000-10-01), DeRobertis et al.
patent: WO 96 14335 (1996-05-01), None
patent: WO 97 48275 (1997-12-01), None
Sambrook, et al., Molecular Cloning: A Laboratory Manual Second Edition vols. 1, 2 and 3. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, NY U.S.A. Nov., 1989, pp. 16.2, 17.2.
Westendorf et al. Wnt signaling in osteoblasts and bone diseases. Gene. Oct. 27, 2004;341:19-39.
P.N. Adler, “Molecular Structure offrizzled,aDrosophilaTissue Polarity Gene”,Genetics126:401-416 (1990).
R.H. Aebersold, et al., “Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis afterin situprotease digestion on nitrocellulose”,Proc. Natl. Acad. Sci. USA84:6970-6974 (1987).
P. Bhanot, et al., “A new member of thefrizzledfamily fromDrosophilafunctions as a Wingless receptor”,Nature382:225-230 (1996).
Bouwmeester T., et al., “Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer.” Nature 382 (6592) 595-601 (1996).
Bowie, et al., “Deciphering the message in protein sequences: tolerance to amino acid substitutions.” Science 247 (4948) 1306-1310 (1990).
G. Camac, et al., “The homebox geneSiamoisis a target of the Wnt dorsalisation pathway and triggers organiser activity in the absence of mesoderm,”Development122:3055-3065 (1996).
S.D.H. Chan, et al., “Two Homologs of theDrosophilaPolarity Genefrizzled(fz) Are Widely Expressed in Mammalian Tissues”,The Jour. of Biol. Chem.267(35):25202-25207 (1992).
J.T. Chang, et al., “Clonding and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium,” Human Mol. Genetics 8(4) 575-583 (1999).
S.C. Chang, et al., “Cartilage-derived Morphogenetic Proteins”,The Jour. of Biol. Chem.269(45):28227-28234 (1994).
J.L. Christian, et al., “Interactions betweenXwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm ofXenopus,” Genes&Development7:13-28 (1993).
Y. Cui, et al., “Xwnt-8b: a maternally expressedXenopus Wntgene with a potential role in establishing the dorsoventral axis”,Development121:2177-2186 (1995).
E.M. De Robertis, et al., “A common plan for dorsoventral patterning in Bilateria,”Nature380:37-40 (1996).
O. Epifano, et al., “Coordinate of the three zona pellucida genes during mouse oogenesis”,Development121:1947-1956 (1995).
A. Eriebacher, et al., “Toward a Molecular Understanding of Skeletal Development”,Cell80:371-378 (1995).
Finch, P.W., et al., “Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action” Proc. Natl Acad Sci USA 94 6770-6775 (1997).
R.M. Harland, “In Situ Hybridization: An Improved Whole-Mount Method for Xenopus Embryos,”Methods Cell Biology36:685-695 (1991).
B. Hoang, et al., “Primary Structure and Tissue Distribution of FRZB, a Novel Protein Related toDrosophilaFrizzled, Suggest a Role in Skeletal Morphogenesis,”The Jour. of Biol. Chemistry271(42):26131-26137 (1996).
S. Hoppler, et al., “Expression of a dominant-negative Wnt blocks induction of MyoD inXenopusembryos,”Genes&Development10:2805-2817 (1996).
K.R. Kao, et al., “The Entire Mesodermal Mantle Behaves as Spemann's Organizer in Dorsoanterior EnhancedXenopus laevisEmbryos,”Developmental Biology127:64-77 (1988).
B.K. Kay, “Injection of Oocytes and Embryos,”Methods Cell Biology36:663-669 (1991).
P. Lemaire, et al., “Expression Cloning ofSiamois,aXenopusHomeobox Gene Expressed in Dorsal-Vegetal Cells of Blastulae and Able to Induce a Complete Secondary Axis,”Cell81:85-94 (1995).
Leyns L., et al., Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer. Cell 88 (6) 747-756 (1997).
F.P. Luyten, et al., “Insulin-like Growth Factors Maintain Steady-State Metabolism of Proteoglycans in Bovine Articular Cartilage Explants”,Archives of Biochem. and Biophys.267(2):416-425 (1988).
F.P. Luyten, et al., “Purification and Partial Amino Acid Sequence of Osteogenin, a Protein Initiating Bone Differentiation”,The Jour. of Biol. Chem.264(23):13377-13380 (1989).
F.P. Luyten, et al., “Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytesin Vitro,” Exper. Cell Res.210:224-229 (1994).
Marieb, E.N., In , Human Anatomy and Physiology. The Benjamin/Cummings Publ. Co., 2ndEd., 373-375 (1992).
Mayr, T., et al., Fritz: a secreted frizzled-related protein that inhibits Wnt activity, Mech. Dev. 63 109-125 (1997).
Melkonyan, H.S., et al., “SARPs: a family of secreted apoptosis-realted proteins,” Proc Natl Acad. Sci. USA, 94 13636-13641 (1997).
R.T. Moon, “In Pursuit of the Functions of theWntFamily of Development Regulators: Insights fromXenopus laevis,” BioEssays15(2):91-97 (1993).
Malcolm Moos, Jr., et al., “Anti-Dorsalizing Morphogenetic Protein is a novel TGF-β homolog expressed in the Spemann organizer,”Development121:4293-4301 (1995).
M. Moos, Jr., et al., “Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support”,The Jour. of Biol. Chem.263(13):6005-6008 (1988).
N. Muthukumaran, et al., “Comparison of Bone Inductive Proteins of Rat and Porcine Bone Matrix”,Biochem. and Biophys. Res. Comm.131(1):37-41 (1985).
J.B. Nardi, et al., “Polarity and gradients in lepidopteran wing epidermis”,J. Embryol. exp. Morph.36(3):489-512 (1976).
Nathan, C., et al., “Cytokines in context.” J of Cell Bio, 113 (5) 981-986, (1991).
R. Nusse, et al., “WntGenes,”Cell69:1073-1087 (1992).
V.M. Paralkar, et al., “Affinity of Osteogenin, and Extracellular Bone Matrix Associated Protein Initiating Bone Differentiation, for Concanavalin A”,Biochem. and Biophys. Res. Comm.160(2):419-424 (1989).
B.A. Parr, et al., “Wntgenes and vertebrate development,”Current Opinion in Genetics and Development4:523-528 (1994).
R.W. Pelton, et al., “Expression of transforming growth factor β2 RNA during murine embryogenesis”,Development106:759-767 (1989).
A. Rattner, et al., “A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors”. Proceedings of the Nat'l Acad of Sci of the USA 94: 2859-2863 (1997).
K. Richter, et al., “Gene expression in the embryonic nervous system ofXenopus laevis”, Proc. Natl. Acad. Sci. USA85:8086-8090 (1988).
S.M. Sato, et al., “Localized and inducible expression ofXenopus-posterior(Xpo), a novel gene active in early frog embryos, encoding a protein with a ‘CCHC’ finger domain,”Development112:747-753 (1991).
J.B. Scales, et al., “Two Distinct Xenopus Genes with Homology to MyoD1 Are Expressed before Somite Formation in Early Embryogenesis,”Molecular and Cellular Biology10(4):1516-1524 (1990).
Shirozu, M., et al., “Characterization of novel secreted and membrane proteins isolated by the signal sequence trap method,” Genomics, 37 273-280 (1996).
J.M.W. Slack, “Inducing factors inXenopusearly embryos,”Current Biology4(2):116-126 (1994).
W.C. Smith, et al., “Injected Xwnt-8 RNA Acts E
Hoang Bang
Luyten Frank P.
Moos, Jr. Malcolm
Wang Shouwen
Knobbe Martens Olson & Bear LLP
Romeo David
The United States of America as represented by the Department of
LandOfFree
Isolation and method of using tissue growth-inducing Frzb... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Isolation and method of using tissue growth-inducing Frzb..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation and method of using tissue growth-inducing Frzb... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3530289