Isolation and identification of control sequences and genes...

Details Isolation and identification of control sequences and genes... Isolation and identification of control sequences and genes...

2006-01-03

2006-01-03

Switzer, Juliet C. (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S091200, C536S023100

Reexamination Certificate

active

06982145

ABSTRACT:
The present invention provides methods and compositions for isolating a nucleic acid molecule fragment comprising at least a portion of a gene, comprising: stimulating at least one cell or at least one nucleus with radiation; cross-linking at least one transcription factor to a nucleic acid molecule in said at least one cell or at least one nucleus with formaldehyde, forming at least one transcription factor-nucleic acid molecule complex; fragmenting said nucleic acid molecule to form at least one transcription factor-nucleic acid molecule fragment complex; and isolating the nucleic acid molecule fragment from said at least one transcription factor-nucleic acid molecule fragment complex to form at least one isolated nucleic acid molecule fragment; wherein said at least one isolated nucleic acid molecule fragment comprises at least a portion of the first exon of a gene whose expression is modulated by said transcription factor; further wherein said at least one isolated nucleic acid molecule fragment comprises at least one transcription factor binding site that is operably linked or in close proximity to said first exon of a gene.

REFERENCES:
patent: 6410233 (2002-06-01), Mercola et al.
de Belle et al. (BioTechniques 29: 162-169, Jul. 2000).
Orlando et al. (Cell, vol. 75, 1187-1198, Dec. 1993).
Hallahan et al. (The Journal of Biological Chemistry, vol. 270, No. 51, p. 30303-30309).

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