Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2006-06-06
2006-06-06
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C536S023100, C435S006120, C435S252100, C546S245000
Reexamination Certificate
active
07056709
ABSTRACT:
Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated fromAcidovorax facilis72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.
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Chauhan Sarita
Dicosimo Robert
Fallon Robert D.
Gavagan John E.
Payne Mark S.
E. I. du Pont de Nemours and Company
Lhulier Christine M.
Meah Md. Younus
Prouty Rebecca E.
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