Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2005-03-22
2005-03-22
Fredman, Jeffrey (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S024300, C435S006120, C435S242000, C435S320100
Reexamination Certificate
active
06870038
ABSTRACT:
Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated fromAcidovorax facilis72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.
REFERENCES:
patent: 4810648 (1989-03-01), Stalker
patent: 5602014 (1997-02-01), Mizummura et al.
patent: 5635391 (1997-06-01), Petre et al.
patent: 5858736 (1999-01-01), DiCosimo et al.
patent: 5935840 (1999-08-01), Anderson
patent: 6066490 (2000-05-01), Di Cosimo et al.
patent: 6413768 (2002-07-01), Galen
patent: 2103616 (1993-08-01), None
patent: WO 01 75077 (2001-10-01), None
http://www.gene-it.com/PDF/GenePASTexperimentV1.pdf, Accessed Dec. 2, 2003.*
Kobayashi, M.,Rhodococcus rhodochrousaliphatic nitrilase gene, complete cds., XP002194942.
Gavagan, J. et al., A Gram-negative Bacterium Producing a Heat-Stable Nitrilase Highly Active on Aliphatic Dinitriles, Applied Microbiology and Biotechnology, Springer Verlag, Berlin, DE, vol. 52, Nov. 1999, pp. 654-659.
Keizou Yamamoto et al: “Purification and Characterization of Nitrilase Responsible for the Enantioselective Hydrolysis from Acinetobacter SP. AK 226”, Agricultural and Biological Chemistry, Japan Soc. For Bioscience, biotechnology and Agrochem. Tokyo, JP, vol. 55, No. 6, pp. 1459-1466, Jun. 1, 1991, XP000226135.
Novo et al,Pseudomanas aeruginosaAliphatic Amidase is related to the Nitrilase/Cyanide Hydratase Enzyme Family and CYS 166 is predicted to be the active site necleophile of the catalytic mechanism, Febs Letters, Elsevier Science Publishers, Amsterdam, NL, vol. 367, No. 3, 1995, pp. 275-279, XP000915286.
Almatawah et al., Characterization of an inducible nitrilase from a thermophilic bacillus, Extremophiles, Springer Verlag, Tokyo, JP, vol. 3, No. 4, Nov. 1999, pp. 283-291, XP001062516.
Pace et al., The nitrilase superfamily: classification, structure and function, Genome Biology, vol. 2, No. 1, Jan. 15, 2001, XP001061987.
Kobayashi et al., Primary Structure of an aliphatic Nitrile-Degrading Enzyme, Aliphatic Nitrilase, fromRhodoccoccus rhodochrousK22 and Expression of its Gene and Identification of its Active Site Residue, Biochemistry, 1992, vol. 31, pp. 9000-9007, XP001062616.
EXTREMOPHILES (1998) 2:207-216.
Kobayashi et al., (Tetrahedron (1990) 46:5587-5590.
Levy-Schil et al., Gene (1995) 161:15-20.
Kobayashi et al., Proc. Nat. Acad. Sci. (1993) 90:247.
Kobayashi et al., J. Biol. Chem. (1992) 267:20746.
Kobayashi et al., Biochem. (1992) 31, 9000.
J. Bacteriology (1990) 172:4807-4815.
Nagasawa et al., Appl. Microbiol. Biotechnol. (1993) 40:189-195.
Cramp et al., Microbiol (1997) 143:2313-2320.
Chauhan Sarita
Dicosimo Robert
Fallon Robert D.
Gavagan John E.
Payne Mark S.
E. I. du Pont de Nemours and Company
Fredman Jeffrey
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