Isolation and expansion of human marrow stromal cells

Details Isolation and expansion of human marrow stromal cells Isolation and expansion of human marrow stromal cells

2008-05-20

2008-05-20

Wehbe′, Anne Marie (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of detaching cells, digesting tissue or establishing...

C435S372000, C435S384000

Reexamination Certificate

active

07374937

ABSTRACT:
The invention includes in vitro methods of inducing and enhancing proliferation of human marrow stromal cells for use in, for example, gene therapy and transplantation methods. The invention also includes a method of assessing the expandability (i.e., proliferative capacity) of human marrow stromal cells. In addition, the invention includes a conditioned medium for enhancing proliferation of human marrow stromal cells.

REFERENCES:
patent: 5766950 (1998-06-01), Greenberger et al.
patent: 5811094 (1998-09-01), Caplan et al.
patent: 5849287 (1998-12-01), Greenberger et al.
patent: 5942225 (1999-08-01), Bruder et al.
Kuznetsov et al. 1997, British Journal of Haematology, 97: 561-570.
Huang et al., 1997, Biotechnology Letters, 19: 89-92.
Kuznetsov et al., 1997, J. Bone and Mineral Res., 12: 1335-1347.
Azizi et al., 1998, PNAS, USA, 95: 3908-3913.
Prockop, 1997, Science, 276: 71-74.
Sekiya et al., 2002, Stem Cells, 20: 530-541.
Ankelsaria, et al., 1987, Proc. Natl. Acad. Sci. USA 84:7681-7685.
Ankelsaria, et al., 1987, Experimental Hematology 15:636-644.
Azizi, et al., 1998, Proc. Natl. Acad. Sci. USA 95:3908-3913.
Beresford, et al., 1992, J. Cell Scoi. 102:341-351.
Bhatia, et al., 1998, Bone Marrow Transplantation 22:87-90.
Bienzle, et al., 1994, Proc. Natl. Acad. Sci. USA, 91:350-354.
Bjornson, et al., 1999, Science 283:534-537.
Byers, et al., 1999, J. Pathol. 187:374-381.
Caplan, 1991, J. Ortho. Res. 9:641-650.
Carpenter, et al., 1999, Exp. Neurol. 158:265-278.
Carter, et al., 1992, Blood 79:356-364.
Castro-Malaspina, et al., 1980, Blood 56:289-301.
Cheng, et al., 1994, Endocrinology 134:277-286.
Chiang, et al., 1999, Human Gene Therapy 10:61-76.
Clark and Keating, 1995, Ann. New York Acad. Sci. 770:70-78.
Eglitis, et al., 1997, Proc. Natl. Sci. USA 94:4080-4085.
Friedenstein, et al., 1976, Experimental Hematology 4:267-274.
Friedenstein, et al., 1987, Cell and Tissue Kinetics 20:263-272.
Glimm, et al., 1999, Blood 97:2161-2168.
Goodell, et al., 1997, Nat. Med. 12:1337-1345.
Gronthos, et al., 1996, J. Hematotheerapy 5:15-23.
Gussoni, et al., 1999, Nature 401:390-393.
Horwitz, et al., 1999, “Transplantibility and therapeutic effects of bone marrow-derived mesenchymal cells in children with severe osteogenesis imperfecta,” Abstracts of the 7thIntl. Meeting on Osteogenesis Imperfecta, Montreal.
Horwitz, et al., 1999, Natl. Med. 5:309-313.
Hou, et al., 1999, Proc. Natl. Acad. Sci. USA 96:7294-7299.
Howlett, et al., 1986, Clin. Orethoped. and Related Res. 213:251-263.
Hurtwitz, et al., 1997, Human Gene Therapy 8:137-156.
Jaiswal, et al., 1997, J. Cell. Bio. 64:295-312.
Joyner, et al., 1997, Bone 21:1-6.
Keating, et al., 1996, Exper. Hematol. 24:1056, Abs. 180.
Kelly, et al., 1998, Endocrinology 139:2622-2628.
Kiefer, 1991, Blood 78:2577-2582.
Kopen, et al., 1999, Proc. Natl. Acad. Sci. USA 96:10711-10716.
Kuznetsov, et al., 1997, Br. J. Haematol. 97:561-570.
Lecka-Czernik, et al., 1999, J. Cell Biochem. 74:357-371.
Long, et al., 1995, J. Clin. Invest. 95:881-887.
Matthews, et al., 1995, Meth. Mol. Biol. 48:273-280.
Mets and Verdonk, 1981, Mech. Aging. Dev. 16:81-89.
Nilsson, et al., 1997, Blood 89:4013-4020.
Nilsson, et al., 1999, J. Exp. Med. 189:729-734.
Owen and Friedenstein, 1988, In: Cell and Molecular Biology of Vertebrate Hard Tissues, pp. 42-60, Ciba Foundation Symposium, Chichester, UK.
Pereira, et al., 1995, Proc. Natl. Acad. Sci. USA 92:4857-4861.
Pereira, et al., 1998. Proc. Natl. Acad. Sci. USA 95:1142-1147.
Piersma, et al., 1985, Experimental Hematology 13:237-243.
Pittenger, et al., 1999, Science 284:143-147.
Rickard, et al., 1994, Dev. Biol. 161:218-228.
Rickard, et al., 1996, J. Bone Miner. Res. 11:312-324.
Satomura, et al., 1998, J. Cell Physiol. 177:426-438.
Simmons and Torok-Storb, 1991, Blood 78:2848-2853.
Simmons, et al., 1991, Blood 78:55-62.
Stanford, et al., 1995, J. Biol. Chem. 270:9420-9428.
Stewart, et al., 1999, J. Bone Miner. Res. 14:1345-1356.
Uzawa, et al., 1999, J. Bone Miner. Res. 14:1272-1280.
Verma, et al. 1997, Nature 389:239-242.
Wakitani, et al., 1994, Muscle and Nerve 18:1417-1426.
Waller, et al., 1995, Blood 85:2422-2435.
Whyte, et al., 1999, “Marrow cell transplatation for infantile hypophosphatasia.” Abstracts of the 7thIntl. Meeting on Osteogenesis Imperfecta, Montreal.
Phinney, et al., 1999, Journal of Cellular Biochemistry, 72:570-585.
Abboud, et al., 1991, vol. 88, August, 470-475.
Kuznetsov, et al., 1997, Journal of Bone and Mineral Research, 12:1335-1347.
Bruder, et al., 1997, Journal of Cellular Biochemistry 64, 278-294.
DiGirolamo, et al. 1999, British Journal of Haematology, vol. 107, No. 2, 275-281.
Colter et al., 2000, PNAS 97:3213-3218.
Javazon et al., 2001, Stem Cells. 19:219-225.
Huss, et al., 1995, Blood, 85:2414-2421.
Huang, et al., 1997, Biotechnology Letters, 19:89-92.

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