Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1992-06-23
1999-10-26
Teng, Sally P.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530395, 530399, 530412, C07K 14705
Patent
active
059731141
ABSTRACT:
Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor.
REFERENCES:
patent: 4833166 (1989-05-01), Grosvenor et al.
Zysk et al., "Cross-Linking of a Growth Horomone Releasing Factor-Binding Protein in Anterior Pituitary Cells," J. Biol. Chem., 261: 16781-16784 (1986).
Velicelebi et al., "Covalent Cross-Linking of Growth Hormone-Releasing Factor to Pituitary Receptors," Endocrinology, 118: 1278-1283 (1986).
Struthers et al., "Nucleotide Regulation of Growth Hormone-Releasing Factor Binding to Rat Pituitary Receptors," Endocrinology, 124: 24-29 (1989).
Christophe et al., "The VIP/PHI/Secretin-Helodermin/Helospectin/GRF Family: Structure-Function Relationship of the Natural Peptides, Their Precursors and Synthetic Analogs as Tested in vitro on Receptors Adenylate Cyclase in a Panel of Tissue Membranes," Peptide Hormones As Prohormones: Processing, Biological Activity, Pharmacology, Ed. Jena Martinez, Publ. Ellis Horwood Lim, Chichester, England (1989).
Laburthe et al., "Molecular Analysis of Vasoactive Intestinal Peptide Receptors: A Comparison With Receptors for VIP-Related Peptides," Ann. NY Acad. Sci., 527: 296-313 (1988).
Froham et al., "Growth Hormone-Releasing Hormone," Endocr Rev., 7: 223-253 (1986).
Seifert et al., "Growth Hormone-Releasing Factor Binding Sites In Rat Anterior Pituitary Membrane Homogenates: Modulation By Glucocorticoids," Endocrinology, 117: 424-426 (1985).
Bilezikjian et al., "Desensitization To Growth Hormone-Releasing Factor (GRF) Is Associated With Down-Regulation of GRF-Binding Sites," Endocrinology, 118: 2045-2052 (1986).
Ishihara et al., "Functional Expression and Tissue Distribution of a Novel Receptor for Vasoactive Intestinal Polypeptide," Neuron, 8:811-819 (1992).
Ishihara et al., "Molecular Cloning and Expression of a cDNA Encoding the Secretin Receptor," EMBO J, 10:1635-1641 (1991).
Lin, et al., "Expression Cloning of an Adenylate Cyclase-Coupled Calcitonin Receptor," Science, 254:1022-1024 (1991).
Juppner, et al., "A G Protein-Linked Receptor For Parathtroid Hormone and Parathyroid Hormone-Related Peptide," Science, 254:1024-1026 (1991).
Frohman, et al., Tissue Distribution and Molecular Heterogeneity of Human Growth Hormone-Releasing Factor in the Transgenic mouse, Endocrinology, 127:2149-2156 (1990).
Paul et al., "Characterization of Receptors for Vasoactive Intestinal Peptide Solubilized From the Lung," J. Biol. Chem., 262:158-162 (1987).
Guijarro et al., "Solubilization of Active and Stable Receptors for Vasoactive Intestinal Peptide from Rat Liver," Regulatory Peptides, 25:37-50 (1989).
Cronin et al., "Biological Activity of a Growth Horomone Releasing Factor Secreted By A Human Tumor," Am. J. Physiol., 244 (Endocrinol Metab) E346-E353 (1983).
Leong et al., "Enumeration of Lactotropes and Somatotropes in Cultured Male and Female Pituitary Cells: Evidence in Favor of a Mammosomatotrope Subpopulation in the Rat," Endocrinology, 116:1371-1378 (1985).
Munson et al., "Ligand: a Versatile Computerized Approach For Characterization of Ligand-Binding Systems," Anal. Biochem., 107:220-239 (1980).
Wessel, et al., "A Methodo for the Quantitative Recovery of Protein in Dilute Solution in the Presence of Detergents and Lipids," Anal. Biochem., 138:141-143 (1984).
Bagnato et al., Gonadotropin-Induced Expression of Receptors for Growth Hormone Releasing Factor in Cultured Granulosa Cells*, Endocrinology, 128:2889-2894 (1991).
Ikuyama et al., "Characterization of Growth Hormone-Releasing Hormone Receptors in Pituitary Adenomas from Patients with Acromegaly," J. Clinical Endocrinology and Metabolism 66:1265-1271 (1988).
Gaylinn et al., "Photoaffinity Crosslinking to GHRH-Receptor Followed by Cyanogen Bromide Cleaviage Produces a labeled 12 kD Glycosylated Peptide," Abstract presented at the Endocrine Society Meeting, Jul. 1993.
Zysk et al., "Purification of the Receptor for Growth Hormone by Streptavidin Sepharose Chromatography," Abstract presented at the Endocrine Society Meeting, Jul. 1993.
Gaylinn et al., "Molecular Cloning and Expression of a Human Anterior Pituitary Receptor for Growth Homrone-Releasing Hormone," Molecular Endocrinology, 7:77-84 (1993).
Kelly E. Mayo, "Molecular Cloning and Expression of a Pituitary-Specific Receptor for Growth Hormone-Releasing Hormone," Molecular Endocrinology, 6:1734-1744 (1992).
Lin et al., "Pit-1-dependent expression of the receptor for growth hormone releasing factor mediates pituitary cell growth," Nature 360:765-768 (Dec. 1992).
Seifert et al., "Binding Sites for Growth Hormone Releasing Factor on Rat Anterior Pituitary Cells," Nature 313:487-489 (Feb. 1985).
Day et al., "Expressionof an antisense viral gene in transgenic tobacco confers resistance to the DNA virus tomato golden mosaic virus," Proc. Natl. Acad. Sci. USA, 88:6721-6725 (Aug. 1991).
Szczylik et al., "Selective inihibition of Leukemia Cell Proliferation by BCR-ABL Antisense Oligodeoxynucleotides," Science 253:562-565 (Aug. 1991).
Han et al., "Inhibition of Moloney murine leukemia virus-induced leukemia in transgenic mice expressing antisense RNA complementary to the retroviral packaging sequences," Proc. Natl. Acad. Sci. USA, 88:4313-3417 (May 1991).
Zhang et al., "Radioimmunoassay of growth hormone-releasing hormone (GHRH) with a polyclonal antibody against synthetic GHRH (1-29)-Gly.sub.4 -Cys-NH.sub.2 : method and clinical studies," Clinica Chimica Acta, 202:243-254 (1991).
Thomas et al., 1990, Methods in Enzymology, 182, 499-520.
Masu et al., 1987, Nature, 329, 836-836.
Yoshida et al., Neuroendocrinology, vol. 52 (51), p. 133, No. P3.106, 1990.
Gearing et al., Embo J., v.8, p. 3667, 1987.
Libert et al., Science, v.244, p. 569, 1989.
Eppler Cecil Mark
Gaylinn Bruce David
Thorner Michael Oliver
Zysk John Ronald
American Cyanamid Company
Teng Sally P.
The University of Virginia Patent Foundation
LandOfFree
Isolation and characterization of the growth hormone releasing h does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Isolation and characterization of the growth hormone releasing h, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation and characterization of the growth hormone releasing h will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-766086