Isolation and characterization of allergen-binding cells for dia

Details Isolation and characterization of allergen-binding cells for dia Isolation and characterization of allergen-binding cells for dia

1998-03-09

1999-06-29

Scheiner, Toni R.

Chemistry: analytical and immunological testing

Involving production or treatment of antibody

Monoclonal antibody

436513, 436526, 436 63, G01N 3353, G01N 33553

Patent

active

059168188

ABSTRACT:
Methods and compositions are provided for the diagnosis of allergen hypersensitivity in a patient. Rare, allergen-specific cells are enriched from a complex cell population, e.g. a patient blood sample. The percentage of blood cells that bind to a particular allergen is less than 0.01%. The allergen-specific cell population is enriched by magnetic cell sorting. In normal blood, the allergen-binding cells are primarily B-cells expressing CD19 and CD21. In blood from allergic patients, an additional population of effector cells, e.g. basophilic granulocytes is labeled by the allergen.

REFERENCES:
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patent: 5595881 (1997-01-01), Kendrick et al.
Irsch et al., Immunotechnology, vol. 1, No. 2, pp. 115-125, Aug. 1995.
Oshiba et al., Clin. Immunol. Immunopath., vol. 72, No. 8, pp. 342-349, Sep. 1994.
Donohue et al., J. Allerg. Clin. Immunol., vol. 95, pp. 587-596, 1995.

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