Isolation and characterization of a novel protease from Streptom

Details Isolation and characterization of a novel protease from Streptom Isolation and characterization of a novel protease from Streptom

1994-07-07

1996-05-14

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

536 232, 536 231, 4353201, 4351723, 435212, 435219, 435 691, 4352523, 935 14, C12N 121, C12N 952, C07H 2104

Patent

active

055166857

ABSTRACT:
The isolation and characterization of a novel polypeptide, designated Protease X, identified while screening for proteases exported into the media by Streptomyces lividans, is described [SEQ ID NO: 2]. Also disclosed is the DNA sequence of the gene encoding this protein [SEQ ID NO: 1]. The polypeptide was found to have proteolytic activity on the substrate succinyl-ala-ala-pro-phe-pNitroanilide, also cleaved by chymotrypsin, subtilisin, and cathepsin G. In addition, methods for purifying the protease and raising antibodies against it are reported. Lastly, the generation of an S. lividans strain deficient in this proteolytic activity is reported. Such a strain may prove useful as a host for production of heterologous protein products.

REFERENCES:
patent: 4865982 (1989-09-01), Burnett, Jr. et al.
Watson, J. D. 1987. in: Molecular Biology of the Gene. 3rd Edition. Benjamin/Cummings Publ. Co., Menlo Park, CA p. 313.
Gibb etal. 1986. Abstr. Ann. Meeting/Am. Soc. For Microbiol. p. 211 abstract K105.
Lichenstein et al. (1988) J. Bacteriology 170(9):3924-3929.
Lichenstein et al. (1992) Gene 111: 125-130.
Duez et al. (1990, Sep. 1 ) FEMS Micro. Lett. 71:215-220.
Henderson et al. (1987) J. Bacteriology 169(8):3778-3784.
Noack et al. (1988) Gene 68:53-62.
Tomich et al. (1988) Antimicrob. Agents Chemo. 32(10):1465-1471.
Woodcock et al. (1989, Feb.) Biochem. Soc. Trans. 17(1):183.
Aretz et al. (1989, Nov.) FEMS Micro. Lett. 65:31-35.

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