Isolation and characterization of a N. crassa silencing...

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C435S325000, C435S252300, C435S254110, C435S419000

Reexamination Certificate

active

07001762

ABSTRACT:
A nucleotide sequence encoding for a protein characterized in that it has a silencing activity and comprises a RNA-dependent RNA polymerase domain is disclosed; furthermore expression vectors suitable for the expression of said sequence in bacteria, plants, animals and fungi are disclosed; the invention refers also to organisms transformed by such vectors.

REFERENCES:
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Cogoni, “Neurospora crassaqde-1 gene, partial”, EMBL Sequence Database, (1999).
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Cogoni et al., “Isolation of quelling-defective (qde) mutants impaired in posttranscriptional transgene-induced gene silencing inNeurospora crassa”, Proc. Nat. Acad. Sci. U.S.A., vol. 94, pp. 10233-10238, (1997).
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