Isolation and bacterial expression of a sesquiterpene synthase C

Details Isolation and bacterial expression of a sesquiterpene synthase C Isolation and bacterial expression of a sesquiterpene synthase C

1998-10-05

1999-12-28

Achutamurthy, Ponnathapura

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

4353201, 435 691, 435 6, 435183, 536 231, 536 232, C12N 500, C12N 1563, C12N 1552

Patent

active

060080434

ABSTRACT:
A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.

REFERENCES:
Salin, F., Pauly, G., Charon, J. and Gleizes, M., J. Plant Phys., 146, pp. 203-209 (1995).
Gershenzon, J., McCaskill, D., Rajaonarivony, J.I.M., Mihaliak, C., Karp, F. and Croteau, R. Anal. Biochem, 200, pp. 130-138 (1992).
Logemann, J., Schell, J. and Willmitzer, L., Anal. Biochem, 163 pp. 16-20, (1987).

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